Monokines inhibiting erythropoietin production in human hepatoma cultures and in isolated perfused rat kidneys

W. Jelkmann; H. Pagel; M. Wolff; J. Fandrey

doi:10.1016/0024-3205(92)90338-P

Monokines inhibiting erythropoietin production in human hepatoma cultures and in isolated perfused rat kidneys

W. Jelkmann^*, H. Pagel, M. Wolff, J. Fandrey

^*Corresponding author for this work

Institute of Physiology

University of Bonn

182 Citations (Scopus)

Abstract

The blood level of erythropoietin (Epo) is often anomalously low in anemic patients with inflammatory or malignant diseases. Therefore, we studied effects of pure recombinant immunomodulatory peptides on Epo formation in cultures of the human hepatoma cell line, HepG2. Interleukin (IL)-1β, IL-1α, and tumor necrosis factor α lowered Epo production with half-maximal inhibition at 2, 5, and 20 U/ml, respectively. IL-6, transforming growth factor β2 and interferon γ did not inhibit. Furthermore, IL-1β (10 U/ml) proved to block Epo formation in isolated serum-free perfused rat kidneys. Proposedly, monokines play a role in the pathogenesis of Epo deficiency in various diseases.

Original language	English
Journal	Life Sciences
Volume	50
Issue number	4
Pages (from-to)	301-308
Number of pages	8
ISSN	0024-3205
DOIs	https://doi.org/10.1016/0024-3205(92)90338-P
Publication status	Published - 1992

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Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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10.1016/0024-3205(92)90338-P

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title = "Monokines inhibiting erythropoietin production in human hepatoma cultures and in isolated perfused rat kidneys",

abstract = "The blood level of erythropoietin (Epo) is often anomalously low in anemic patients with inflammatory or malignant diseases. Therefore, we studied effects of pure recombinant immunomodulatory peptides on Epo formation in cultures of the human hepatoma cell line, HepG2. Interleukin (IL)-1β, IL-1α, and tumor necrosis factor α lowered Epo production with half-maximal inhibition at 2, 5, and 20 U/ml, respectively. IL-6, transforming growth factor β2 and interferon γ did not inhibit. Furthermore, IL-1β (10 U/ml) proved to block Epo formation in isolated serum-free perfused rat kidneys. Proposedly, monokines play a role in the pathogenesis of Epo deficiency in various diseases.",

author = "W. Jelkmann and H. Pagel and M. Wolff and J. Fandrey",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaff (DFG Je 95/6). The authors would like to thank Mrs. Elisabeth Mauch and Mrs. Karin Piccardi for expert technical assistance and Mrs. Ursula Isenberg for secretarial assistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

doi = "10.1016/0024-3205(92)90338-P",

language = "English",

volume = "50",

pages = "301--308",

journal = "Life Sciences",

issn = "0024-3205",

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TY - JOUR

T1 - Monokines inhibiting erythropoietin production in human hepatoma cultures and in isolated perfused rat kidneys

AU - Jelkmann, W.

AU - Pagel, H.

AU - Wolff, M.

AU - Fandrey, J.

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaff (DFG Je 95/6). The authors would like to thank Mrs. Elisabeth Mauch and Mrs. Karin Piccardi for expert technical assistance and Mrs. Ursula Isenberg for secretarial assistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1992

Y1 - 1992

N2 - The blood level of erythropoietin (Epo) is often anomalously low in anemic patients with inflammatory or malignant diseases. Therefore, we studied effects of pure recombinant immunomodulatory peptides on Epo formation in cultures of the human hepatoma cell line, HepG2. Interleukin (IL)-1β, IL-1α, and tumor necrosis factor α lowered Epo production with half-maximal inhibition at 2, 5, and 20 U/ml, respectively. IL-6, transforming growth factor β2 and interferon γ did not inhibit. Furthermore, IL-1β (10 U/ml) proved to block Epo formation in isolated serum-free perfused rat kidneys. Proposedly, monokines play a role in the pathogenesis of Epo deficiency in various diseases.

AB - The blood level of erythropoietin (Epo) is often anomalously low in anemic patients with inflammatory or malignant diseases. Therefore, we studied effects of pure recombinant immunomodulatory peptides on Epo formation in cultures of the human hepatoma cell line, HepG2. Interleukin (IL)-1β, IL-1α, and tumor necrosis factor α lowered Epo production with half-maximal inhibition at 2, 5, and 20 U/ml, respectively. IL-6, transforming growth factor β2 and interferon γ did not inhibit. Furthermore, IL-1β (10 U/ml) proved to block Epo formation in isolated serum-free perfused rat kidneys. Proposedly, monokines play a role in the pathogenesis of Epo deficiency in various diseases.

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DO - 10.1016/0024-3205(92)90338-P

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SN - 0024-3205

VL - 50

SP - 301

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JO - Life Sciences

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ER -

Monokines inhibiting erythropoietin production in human hepatoma cultures and in isolated perfused rat kidneys

Abstract

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