Monocytic angiotensin and endothelin receptor imbalance modulate secretion of the profibrotic chemokine ligand 18

Judith Rademacher; Angela Kill; Kathrin Mattat; Duska Dragun; Elise Siegert; Jeannine Günther; Gabriela Riemekasten

doi:10.3899/jrheum.150474

Monocytic angiotensin and endothelin receptor imbalance modulate secretion of the profibrotic chemokine ligand 18

Judith Rademacher, Angela Kill, Kathrin Mattat, Duska Dragun, Elise Siegert, Jeannine Günther, Gabriela Riemekasten^*

^*Corresponding author for this work

Clinic of Rheumatology

Charite - Universitatsmedizin Berlin

11 Citations (Scopus)

Abstract

Objective. To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance. Methods. Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA. Results. Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion. Conclusion. Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.

Original language	English
Journal	Journal of Rheumatology
Volume	43
Issue number	3
Pages (from-to)	587-591
Number of pages	5
ISSN	0315-162X
DOIs	https://doi.org/10.3899/jrheum.150474
Publication status	Published - 03.2016

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3899/jrheum.150474

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title = "Monocytic angiotensin and endothelin receptor imbalance modulate secretion of the profibrotic chemokine ligand 18",

abstract = "Objective. To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance. Methods. Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA. Results. Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion. Conclusion. Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.",

author = "Judith Rademacher and Angela Kill and Kathrin Mattat and Duska Dragun and Elise Siegert and Jeannine G{\"u}nther and Gabriela Riemekasten",

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doi = "10.3899/jrheum.150474",

language = "English",

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T1 - Monocytic angiotensin and endothelin receptor imbalance modulate secretion of the profibrotic chemokine ligand 18

AU - Rademacher, Judith

AU - Kill, Angela

AU - Mattat, Kathrin

AU - Dragun, Duska

AU - Siegert, Elise

AU - Günther, Jeannine

AU - Riemekasten, Gabriela

PY - 2016/3

Y1 - 2016/3

N2 - Objective. To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance. Methods. Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA. Results. Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion. Conclusion. Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.

AB - Objective. To assess monocytic expression and ratio of angiotensin and endothelin receptors in systemic sclerosis (SSc) and their functional relevance. Methods. Receptor expression was measured by flow cytometry. Chemokine ligand 18 (CCL18) concentration in supernatants of peripheral blood mononuclear cells stimulated with immunoglobulin G was measured by ELISA. Results. Monocytes of patients with SSc presented an increased angiotensin II Type 1 receptor (AT1R)/AT2R ratio compared with those of healthy donors. Patients with lung fibrosis and patients with high modified Rodnan skin score showed a reduced endothelin 1 Type A receptor (ETAR)/ETBR ratio. High AT1R/AT2R, but low ETAR/ETBR ratios corresponded to higher CCL18 secretion. Conclusion. Altered angiotensin and endothelin receptor ratios observed in SSc influence autoantibody-mediated effects such as secretion of profibrotic CCL18.

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Monocytic angiotensin and endothelin receptor imbalance modulate secretion of the profibrotic chemokine ligand 18

Abstract

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