Monoclonal antibody WGM1 directed against proteinase 3: An immunohistochemical marker for naphthol ASD chloroacetate

Matthias G. Braun; Elena Csernok; Wiebke Rögener-Schwarz; Wolf Dieter Ludwig; Hans Konrad Müller-Hermelink; Wolfgang L. Gross; Alfred C. Feller

doi:10.1002/(SICI)1099-1069(199606)14:2<83::AID-HON570>3.0.CO;2-N

Monoclonal antibody WGM1 directed against proteinase 3: An immunohistochemical marker for naphthol ASD chloroacetate

Matthias G. Braun^*, Elena Csernok, Wiebke Rögener-Schwarz, Wolf Dieter Ludwig, Hans Konrad Müller-Hermelink, Wolfgang L. Gross, Alfred C. Feller

^*Corresponding author for this work

5 Citations (Scopus)

Abstract

Enzyme histochemistry for naphthol ASD chloroacetate (NASDCA, 'Leder's stain') is used to identify the granulocyte lineage ranging from promyelocytes to mature neutrophils and is an additional tool for the characterization of leukemias. We demonstrated for the first time that NASDCA activity can be detected by routine immunohistochemistry and immunocytochemistry using the monoclonal antibody WGM1 directed against proteinase 3 (PR3; synonyms: Wegener's autoantigen, myeloblastin). Immunohisto- and immunocytochemical staining with WGM1 against PR3 and enzyme histochemistry for NASDCA produced identical staining patterns in normal myelomonocytic cells and cells of myeloid leukemia. This was additionally proven by double immunostainings. We have also shown that PR3 is one of the specific proteinases responsible for hydrolysis of NASDCA.

Original language	English
Journal	Hematological Oncology
Volume	14
Issue number	2
Pages (from-to)	83-90
Number of pages	8
ISSN	0278-0232
DOIs	https://doi.org/10.1002/(SICI)1099-1069(199606)14:2<83::AID-HON570>3.0.CO;2-N
Publication status	Published - 06.1996

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10.1002/(SICI)1099-1069(199606)14:2<83::AID-HON570>3.0.CO;2-N

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title = "Monoclonal antibody WGM1 directed against proteinase 3: An immunohistochemical marker for naphthol ASD chloroacetate",

abstract = "Enzyme histochemistry for naphthol ASD chloroacetate (NASDCA, 'Leder's stain') is used to identify the granulocyte lineage ranging from promyelocytes to mature neutrophils and is an additional tool for the characterization of leukemias. We demonstrated for the first time that NASDCA activity can be detected by routine immunohistochemistry and immunocytochemistry using the monoclonal antibody WGM1 directed against proteinase 3 (PR3; synonyms: Wegener's autoantigen, myeloblastin). Immunohisto- and immunocytochemical staining with WGM1 against PR3 and enzyme histochemistry for NASDCA produced identical staining patterns in normal myelomonocytic cells and cells of myeloid leukemia. This was additionally proven by double immunostainings. We have also shown that PR3 is one of the specific proteinases responsible for hydrolysis of NASDCA.",

author = "Braun, {Matthias G.} and Elena Csernok and Wiebke R{\"o}gener-Schwarz and Ludwig, {Wolf Dieter} and M{\"u}ller-Hermelink, {Hans Konrad} and Gross, {Wolfgang L.} and Feller, {Alfred C.}",

year = "1996",

month = jun,

doi = "10.1002/(SICI)1099-1069(199606)14:2<83::AID-HON570>3.0.CO;2-N",

language = "English",

volume = "14",

pages = "83--90",

journal = "Hematological Oncology",

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T1 - Monoclonal antibody WGM1 directed against proteinase 3: An immunohistochemical marker for naphthol ASD chloroacetate

AU - Braun, Matthias G.

AU - Csernok, Elena

AU - Rögener-Schwarz, Wiebke

AU - Ludwig, Wolf Dieter

AU - Müller-Hermelink, Hans Konrad

AU - Gross, Wolfgang L.

AU - Feller, Alfred C.

PY - 1996/6

Y1 - 1996/6

N2 - Enzyme histochemistry for naphthol ASD chloroacetate (NASDCA, 'Leder's stain') is used to identify the granulocyte lineage ranging from promyelocytes to mature neutrophils and is an additional tool for the characterization of leukemias. We demonstrated for the first time that NASDCA activity can be detected by routine immunohistochemistry and immunocytochemistry using the monoclonal antibody WGM1 directed against proteinase 3 (PR3; synonyms: Wegener's autoantigen, myeloblastin). Immunohisto- and immunocytochemical staining with WGM1 against PR3 and enzyme histochemistry for NASDCA produced identical staining patterns in normal myelomonocytic cells and cells of myeloid leukemia. This was additionally proven by double immunostainings. We have also shown that PR3 is one of the specific proteinases responsible for hydrolysis of NASDCA.

AB - Enzyme histochemistry for naphthol ASD chloroacetate (NASDCA, 'Leder's stain') is used to identify the granulocyte lineage ranging from promyelocytes to mature neutrophils and is an additional tool for the characterization of leukemias. We demonstrated for the first time that NASDCA activity can be detected by routine immunohistochemistry and immunocytochemistry using the monoclonal antibody WGM1 directed against proteinase 3 (PR3; synonyms: Wegener's autoantigen, myeloblastin). Immunohisto- and immunocytochemical staining with WGM1 against PR3 and enzyme histochemistry for NASDCA produced identical staining patterns in normal myelomonocytic cells and cells of myeloid leukemia. This was additionally proven by double immunostainings. We have also shown that PR3 is one of the specific proteinases responsible for hydrolysis of NASDCA.

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DO - 10.1002/(SICI)1099-1069(199606)14:2<83::AID-HON570>3.0.CO;2-N

M3 - Journal articles

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JO - Hematological Oncology

JF - Hematological Oncology

IS - 2

ER -

Monoclonal antibody WGM1 directed against proteinase 3: An immunohistochemical marker for naphthol ASD chloroacetate

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