Molecular Recognition of UDP-Gal by β-1,4-Galactosyltransferase T1

Thorsten Biet; Thomas Peters

doi:10.1002/1521-3773(20011119)40:22<4189::AID-ANIE4189>3.0.CO;2-A

Molecular Recognition of UDP-Gal by β-1,4-Galactosyltransferase T1

Thorsten Biet, Thomas Peters^*

^*Corresponding author for this work

Institute of Chemistry and Metabolomics

32 Citations (Scopus)

Abstract

Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.

Original language	English
Journal	Angewandte Chemie - International Edition
Volume	40
Issue number	22
Pages (from-to)	4189-4192
Number of pages	4
ISSN	1433-7851
DOIs	https://doi.org/10.1002/1521-3773(20011119)40:22<4189::AID-ANIE4189>3.0.CO;2-A
Publication status	Published - 19.11.2001

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Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1002/1521-3773(20011119)40:22<4189::AID-ANIE4189>3.0.CO;2-A

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abstract = "Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.",

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AU - Biet, Thorsten

AU - Peters, Thomas

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N2 - Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.

AB - Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.

UR - https://www.scopus.com/pages/publications/0035915156

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M3 - Journal articles

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JF - Angewandte Chemie - International Edition

IS - 22

ER -

Molecular Recognition of UDP-Gal by β-1,4-Galactosyltransferase T1

Abstract

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