TY - JOUR
T1 - Molecular Recognition of UDP-Gal by β-1,4-Galactosyltransferase T1
AU - Biet, Thorsten
AU - Peters, Thomas
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001/11/19
Y1 - 2001/11/19
N2 - Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.
AB - Saturation transfer difference (STD) NMR experiments reveal the binding epitopes of UDP-Gal and UDP-Glc bound to the glycosyltransferase β4Gal-T1 (see picture). Whereas the enzyme recognizes the galactose residue in UDP-Gal, it does not make any close contacts with the glucose residue in UDP-Glc. This observation explains why β4Gal-T1 binds to UDP-Glc but is unable to transfer glucose to an acceptor substrate.
UR - http://www.scopus.com/inward/record.url?scp=0035915156&partnerID=8YFLogxK
U2 - 10.1002/1521-3773(20011119)40:22<4189::AID-ANIE4189>3.0.CO;2-A
DO - 10.1002/1521-3773(20011119)40:22<4189::AID-ANIE4189>3.0.CO;2-A
M3 - Journal articles
AN - SCOPUS:0035915156
VL - 40
SP - 4189
EP - 4192
JO - Angewandte Chemie (International Edition in English)
JF - Angewandte Chemie (International Edition in English)
SN - 0570-0833
IS - 22
ER -