Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene

Teruyuki Niimi, Ken Sahara, Hiroyuki Oshima, Yuji Yasukochi, Kazuho Ikeo, Walther Traut*

*Corresponding author for this work
28 Citations (Scopus)

Abstract

We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.

Original languageEnglish
JournalGenome
Volume49
Issue number3
Pages (from-to)263-268
Number of pages6
ISSN0831-2796
DOIs
Publication statusPublished - 01.03.2006

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