Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene

Teruyuki Niimi; Ken Sahara; Hiroyuki Oshima; Yuji Yasukochi; Kazuho Ikeo; Walther Traut

doi:10.1139/g05-108

Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene

Teruyuki Niimi, Ken Sahara, Hiroyuki Oshima, Yuji Yasukochi, Kazuho Ikeo, Walther Traut^*

^*Corresponding author for this work

Institute of Biology

28 Citations (Scopus)

Abstract

We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F₂ panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.

Original language	English
Journal	Genome
Volume	49
Issue number	3
Pages (from-to)	263-268
Number of pages	6
ISSN	0831-2796
DOIs	https://doi.org/10.1139/g05-108
Publication status	Published - 01.03.2006

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title = "Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene",

abstract = "We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.",

author = "Teruyuki Niimi and Ken Sahara and Hiroyuki Oshima and Yuji Yasukochi and Kazuho Ikeo and Walther Traut",

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doi = "10.1139/g05-108",

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T1 - Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene

AU - Niimi, Teruyuki

AU - Sahara, Ken

AU - Oshima, Hiroyuki

AU - Yasukochi, Yuji

AU - Ikeo, Kazuho

AU - Traut, Walther

PY - 2006/3/1

Y1 - 2006/3/1

N2 - We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.

AB - We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.

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DO - 10.1139/g05-108

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SN - 0831-2796

VL - 49

SP - 263

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JO - Genome

JF - Genome

IS - 3

ER -

Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene

Abstract

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