ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: Catalytic properties and mutation analysis

Bernd Tiemann, Reinhard Depping, Egle Gineikiene, Laura Kaliniene, Rimas Nivinskas, Wolfgang Rüger*

*Corresponding author for this work
22 Citations (Scopus)


Bacteriophage T4 encodes three ADP-ribosyltransferases, Alt, ModA, and ModB. These enzymes participate in the regulation of the T4 replication cycle by ADP-ribosylating a defined set of host proteins. In order to obtain a better understanding of the phage-host interactions and their consequences for regulating the T4 replication cycle, we studied cloning, overespression, and characterization of purified ModA and ModB enzymes. Site-directed mutagenesis confirmed that amino acids, as deduced from secondary structure alignments, are indeed decisive for the activity of the enzymes, implying that the transfer reaction follows the Sn1-type reaction scheme proposed for this class of enzymes. In vitro transcription assays performed with Alt- and ModA-modified RNA polymerases demonstrated that the Alt-ribosylated polymerase enhances transcription from T4 early promoters on a T4 DNA template, whereas the transcriptional activity of ModA-modified polymerase, without the participation of T4-encoded auxiliary proteins for middle mode or late transcription, is reduced. The results presented here support the conclusion that ADP-ribosylation of RNA polymerase and of other host proteins allows initial phage-directed mRNA synthesis reactions to escape from host control. In contrast, subsequent modification of the other cellular target proteins limits transcription from phage early genes and participates in redirecting transcription to phage middle and late genes.

Original languageEnglish
JournalJournal of Bacteriology
Issue number21
Pages (from-to)7262-7272
Number of pages11
Publication statusPublished - 11.2004

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)


Dive into the research topics of 'ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: Catalytic properties and mutation analysis'. Together they form a unique fingerprint.

Cite this