TY - JOUR
T1 - Mitochondrial OXPHOS functions in R1H rhabdomyosarcoma and skeletal muscles of the rat
AU - Kuhnt, Thomas
AU - Pelz, Tanja
AU - Qu, Xiaoying
AU - Hänsgen, Gabriele
AU - Dunst, Jürgen
AU - Gellerich, Frank Norbert
N1 - Funding Information:
Acknowledgment This work was partly supported by a grant of the Deutsche Forschungsgemeinschaft (Grant number Ge 664/1-1).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/6
Y1 - 2007/6
N2 - The aim of the study was to determinate mitochondrial oxidative phosphorylation (OXPHOS) functions in rat rhabdomyosarcoma R1H (R1H) and rat skeletal muscles. For that purpose skinned fiber technique and multiple substrate inhibitor titration were adapted to tumor samples. In our animal tumor model (R1H) functional abnormalities of OXPHOS were found compared to skeletal muscles. In R1H the state 3 respiration of pyruvate + malate was decreased: 0.56 ± 0.28 nmol O2/mg/min versus 2.32 ± 1.19 nmol O 2/mg/min, P < 0.001, whereas the state 3 respiration of succinate + rotenone was increased: 36 ± 14% versus 19 ± 11%, P < 0.001. In R1H the rotenone-insensitive respiration reached higher levels than the antimycin A-insensitive respiration, whereas in normal muscles the converse was observed. Additionally, the obvious difference between the CAT- and the antimycin A-independent respiration indicates an increased part of leak respiration in R1H. By now, the high feasibility of these techniques is appreciated for the investigation of muscles and prospectively for tumors, too.
AB - The aim of the study was to determinate mitochondrial oxidative phosphorylation (OXPHOS) functions in rat rhabdomyosarcoma R1H (R1H) and rat skeletal muscles. For that purpose skinned fiber technique and multiple substrate inhibitor titration were adapted to tumor samples. In our animal tumor model (R1H) functional abnormalities of OXPHOS were found compared to skeletal muscles. In R1H the state 3 respiration of pyruvate + malate was decreased: 0.56 ± 0.28 nmol O2/mg/min versus 2.32 ± 1.19 nmol O 2/mg/min, P < 0.001, whereas the state 3 respiration of succinate + rotenone was increased: 36 ± 14% versus 19 ± 11%, P < 0.001. In R1H the rotenone-insensitive respiration reached higher levels than the antimycin A-insensitive respiration, whereas in normal muscles the converse was observed. Additionally, the obvious difference between the CAT- and the antimycin A-independent respiration indicates an increased part of leak respiration in R1H. By now, the high feasibility of these techniques is appreciated for the investigation of muscles and prospectively for tumors, too.
UR - http://www.scopus.com/inward/record.url?scp=34248215099&partnerID=8YFLogxK
U2 - 10.1007/s11064-006-9254-0
DO - 10.1007/s11064-006-9254-0
M3 - Journal articles
C2 - 17273927
AN - SCOPUS:34248215099
SN - 0364-3190
VL - 32
SP - 973
EP - 980
JO - Neurochemical Research
JF - Neurochemical Research
IS - 6
ER -
