Abstract
In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.
| Original language | English |
|---|---|
| Journal | Acta Chimica Slovenica |
| Volume | 62 |
| Issue number | 4 |
| Pages (from-to) | 761-767 |
| Number of pages | 7 |
| ISSN | 1318-0207 |
| DOIs | |
| Publication status | Published - 2015 |
Funding
This work was funded by the Ministry of Science and Technological Development, Republic of Serbia, grant 173014 "Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology". The authors thank the Wellcome Trust and the EU in the context of the EU-NMR grant (RII3-026145) for supporting the HWBNMR facility in Birmingham. The authors thank the EU for supporting S.T. in the context of the MOTET Marie Curie project. We thank Dr. Edward Petri for editing this manuscript for English grammar and content.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
