TY - JOUR
T1 - Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)
AU - Purać, Jelena
AU - Kojić, Danijela
AU - Popović, Željko D.
AU - Vukašinović, Elvira
AU - Tiziani, Stefano
AU - Günther, Ulrich L.
AU - Grubor-Lajšić, Gordana
N1 - Funding Information:
This work was funded by the Ministry of Science and Technological Development, Republic of Serbia, grant 173014 "Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology". The authors thank the Wellcome Trust and the EU in the context of the EU-NMR grant (RII3-026145) for supporting the HWBNMR facility in Birmingham. The authors thank the EU for supporting S.T. in the context of the MOTET Marie Curie project. We thank Dr. Edward Petri for editing this manuscript for English grammar and content.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2015
Y1 - 2015
N2 - In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.
AB - In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.
UR - http://www.scopus.com/inward/record.url?scp=84954149211&partnerID=8YFLogxK
U2 - 10.17344/acsi.2015.1370
DO - 10.17344/acsi.2015.1370
M3 - Journal articles
C2 - 26680702
AN - SCOPUS:84954149211
SN - 1318-0207
VL - 62
SP - 761
EP - 767
JO - Acta Chimica Slovenica
JF - Acta Chimica Slovenica
IS - 4
ER -
