Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Claudia Gey; Karsten Seeger

doi:10.1007/978-1-4939-6670-7_15

Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Claudia Gey, Karsten Seeger^*

^*Corresponding author for this work

Institute of Chemistry and Metabolomics

7 Citations (Scopus)

Abstract

Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

Original language	English
Journal	Methods in Molecular Biology
Volume	1534
Pages (from-to)	155-163
Number of pages	9
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-4939-6670-7_15
Publication status	Published - 01.01.2017

Funding

The work was financially supported by the DFG Excellence Cluster "Inflammation at Interfaces" and the "Doktor Robert Pfleger Stiftung Bamberg." D. Zapf is acknowledged for technical assistance.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-6670-7_15

Cite this

@article{e94aa718a01f4ce29639073e4704f83c,

title = "Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence",

abstract = "Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.",

author = "Claudia Gey and Karsten Seeger",

year = "2017",

month = jan,

day = "1",

doi = "10.1007/978-1-4939-6670-7\_15",

language = "English",

volume = "1534",

pages = "155--163",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

AU - Gey, Claudia

AU - Seeger, Karsten

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

AB - Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

UR - https://www.scopus.com/pages/publications/84994533203

U2 - 10.1007/978-1-4939-6670-7_15

DO - 10.1007/978-1-4939-6670-7_15

M3 - Journal articles

C2 - 27812877

AN - SCOPUS:84994533203

SN - 1064-3745

VL - 1534

SP - 155

EP - 163

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Abstract

Funding

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this