Abstract
Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.
| Original language | English |
|---|---|
| Journal | Methods in Molecular Biology |
| Volume | 1534 |
| Pages (from-to) | 155-163 |
| Number of pages | 9 |
| ISSN | 1064-3745 |
| DOIs | |
| Publication status | Published - 01.01.2017 |
Funding
The work was financially supported by the DFG Excellence Cluster "Inflammation at Interfaces" and the "Doktor Robert Pfleger Stiftung Bamberg." D. Zapf is acknowledged for technical assistance.
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
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