Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Claudia Gey; Karsten Seeger

doi:10.1007/978-1-4939-6670-7_15

Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Claudia Gey, Karsten Seeger^*

^*Corresponding author for this work

Institute of Chemistry and Metabolomics

2 Citations (Scopus)

Abstract

Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

Original language	English
Journal	Methods in Molecular Biology
Volume	1534
Pages (from-to)	155-163
Number of pages	9
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-4939-6670-7_15
Publication status	Published - 01.01.2017

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10.1007/978-1-4939-6670-7_15

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AB - Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

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Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence

Abstract

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