Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay

J. Kekow, K. Ulrichs, M. Muller-Ruchholtz, W. L. Gross

72 Citations (Scopus)

Abstract

Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELISA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/ml IRI (1 ng/ml ~ 23.4 μU/ml ~ 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of ≤15%.

Original languageEnglish
JournalDiabetes
Volume37
Issue number3
Pages (from-to)321-326
Number of pages6
ISSN0012-1797
DOIs
Publication statusPublished - 1988

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

Fingerprint

Dive into the research topics of 'Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay'. Together they form a unique fingerprint.

Cite this