TY - JOUR
T1 - Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay
AU - Kekow, J.
AU - Ulrichs, K.
AU - Muller-Ruchholtz, M.
AU - Gross, W. L.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELISA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/ml IRI (1 ng/ml ~ 23.4 μU/ml ~ 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of ≤15%.
AB - Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELISA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/ml IRI (1 ng/ml ~ 23.4 μU/ml ~ 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of ≤15%.
UR - http://www.scopus.com/inward/record.url?scp=0023870117&partnerID=8YFLogxK
U2 - 10.2337/diab.37.3.321
DO - 10.2337/diab.37.3.321
M3 - Journal articles
C2 - 3286333
AN - SCOPUS:0023870117
SN - 0012-1797
VL - 37
SP - 321
EP - 326
JO - Diabetes
JF - Diabetes
IS - 3
ER -
