Maternally inherited differences within mitochondrial Complex I control murine healthspan.

Misa Hirose, Paul Schilf, Kim Zarse, Hauke Busch, Georg Fuellen, Olaf Joehren, Ruediger Koehling, Inke R Koenig, Barbara Richer, Jan Rupp, Markus Schwaninger, Karsten Seeger, Christian Sina, Michael Ristow, Saleh M Ibrahim


Mitochondrial complex I, the largest enzyme complex of the mitochondrial oxidative phosphorylation machinery, has been proposed to contribute to a variety of age-related pathological alterations as well as longevity. The enzyme complex-consisting proteins are encoded by both nuclear (nDNA) and mitochondrial DNA (mtDNA). While some association studies of mtDNA-encoded complex I genes and lifespan in humans have been reported, experimental evidence and the functional consequence of such variants is limited to studies using invertebrate models. Here, we present experimental evidence that a homoplasmic mutation in the mitochondrially encoded complex I gene mt-Nd2 modulates lifespan by altering cellular tryptophan levels and, consequently, ageing-related pathways in mice. A conplastic mouse strain carrying a mutation at m.4738C>A in mt-Nd2 lived significantly shorter than the controls did. The same mutation led to a higher susceptibility to glucose intolerance induced by high-fat diet feeding. These phenotypes were not observed in mice carrying a mutation in another mtDNA-encoded complex I gene, mt-Nd5, suggesting the functional relevance of particular mutations in complex I to ageing and age-related diseases.
Original languageGerman
Pages (from-to)472092
Publication statusPublished - 2018

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