TY - JOUR
T1 - Mass spectrometric comparison of HPV-positive and hpv-negative oropharyngeal cancer
AU - Wurlitzer, Marcus
AU - Möckelmann, Nikolaus
AU - Kriegs, Malte
AU - Vens, Maren
AU - Omidi, Maryam
AU - Hoffer, Konstantin
AU - von Bargen, Clara
AU - Möller-Koop, Christina
AU - Witt, Melanie
AU - Droste, Conrad
AU - Oetting, Agnes
AU - Petersen, Hannes
AU - Busch, Chia Jung
AU - Münscher, Adrian
AU - Schlüter, Hartmut
AU - Clauditz, Till Sebastian
AU - Rieckmann, Thorsten
PY - 2020/6
Y1 - 2020/6
N2 - Squamous cell carcinoma of the head and neck (HNSCC) consist of two distinct biological entities. While the numbers of classical, tobacco-induced HNSCC are declining, tumors caused by human papillomavirus (HPV) infection are increasing in many countries. HPV-positive HNSCC mostly arise in the oropharynx and are characterized by an enhanced sensitivity towards radiotherapy and a favorable prognosis. To identify molecular differences between both entities on the protein level, we conducted a mass spectrometric comparison of eight HPV-positive and nine HPV-negative oropharyngeal tumors (OPSCC). Overall, we identified 2051 proteins, of which 31 were found to be differentially expressed. Seventeen of these can be assorted to three functional groups, namely DNA replication, nuclear architecture and cytoskeleton regulation, with the differences in the last group potentially reflecting an enhanced migratory and invasive capacity. Furthermore, a number of identified proteins have been described to directly impact on DNA double-strand break repair or radiation sensitivity (e.g., SLC3A2, cortactin, RBBP4, Numa1), offering explanations for the differential prognosis. The unequal expression of three proteins (SLC3A2, MCM2 and lamin B1) was confirmed by immunohistochemical staining using a tissue microarray containing 205 OPSCC samples. The expression levels of SLC3A2 and lamin B1 were found be of prognostic relevance in patients with HPV-positive and HPV-negative OPSCC, respectively.
AB - Squamous cell carcinoma of the head and neck (HNSCC) consist of two distinct biological entities. While the numbers of classical, tobacco-induced HNSCC are declining, tumors caused by human papillomavirus (HPV) infection are increasing in many countries. HPV-positive HNSCC mostly arise in the oropharynx and are characterized by an enhanced sensitivity towards radiotherapy and a favorable prognosis. To identify molecular differences between both entities on the protein level, we conducted a mass spectrometric comparison of eight HPV-positive and nine HPV-negative oropharyngeal tumors (OPSCC). Overall, we identified 2051 proteins, of which 31 were found to be differentially expressed. Seventeen of these can be assorted to three functional groups, namely DNA replication, nuclear architecture and cytoskeleton regulation, with the differences in the last group potentially reflecting an enhanced migratory and invasive capacity. Furthermore, a number of identified proteins have been described to directly impact on DNA double-strand break repair or radiation sensitivity (e.g., SLC3A2, cortactin, RBBP4, Numa1), offering explanations for the differential prognosis. The unequal expression of three proteins (SLC3A2, MCM2 and lamin B1) was confirmed by immunohistochemical staining using a tissue microarray containing 205 OPSCC samples. The expression levels of SLC3A2 and lamin B1 were found be of prognostic relevance in patients with HPV-positive and HPV-negative OPSCC, respectively.
UR - http://www.scopus.com/inward/record.url?scp=85086396216&partnerID=8YFLogxK
U2 - 10.3390/cancers12061531
DO - 10.3390/cancers12061531
M3 - Journal articles
AN - SCOPUS:85086396216
VL - 12
SP - 1
EP - 22
JO - Cancers
JF - Cancers
IS - 6
M1 - 1531
ER -
