TY - JOUR
T1 - Macrophage cholesterol efflux correlates with lipoprotein subclass distribution and risk of obstructive coronary artery disease in patients undergoing coronary angiography
AU - Linsel-Nitschke, Patrick
AU - Jansen, Henning
AU - Aherrarhou, Zouhair
AU - Belz, Stefanie
AU - Mayer, Björn
AU - Lieb, Wolfgang
AU - Huber, Fritz
AU - Kremer, Werner
AU - Kalbitzer, Hans Robert
AU - Erdmann, Jeanette
AU - Schunkert, Heribert
PY - 2009/5/6
Y1 - 2009/5/6
N2 - Background. Studies in patients with low HDL have suggested that impaired cellular cholesterol efflux is a heritable phenotype increasing atherosclerosis risk. Less is known about the association of macrophage cholesterol efflux with lipid profiles and CAD risk in normolipidemic subjects. We have therefore measured macrophage cholesterol efflux in142 normolipidemic subjects undergoing coronary angiography. Methods. Monocytes isolated from blood samples of patients scheduled for cardiac catheterization were differentiated into macrophages over seven days. Isotopic cholesterol efflux to exogenously added apolipoprotein A-I and HDL2 was measured. Quantitative cholesterol efflux from macrophages was correlated with lipoprotein subclass distribution in plasma from the same individuals measured by NMR-spectroscopy of lipids and with the extent of coronary artery disease seen on coronary angiography. Results. Macrophage cholesterol efflux was positively correlated with particle concentration of smaller HDL and LDL particles but not with total plasma concentrations of HDL or LDL-cholesterol. We observed an inverse relationship between macrophage cholesterol efflux and the concntration of larger and triglyceride rich particles (VLDL, chylomicrons). Subjects with significant stenosis on coronary angiography had lower cholesterol efflux from macrophages compared to individuals without significant stenosis (adjusted p = 0.02). Conclusion. Macrophage cholesterol efflux is inversely correlated with lipoprotein particle size and risk of CAD.
AB - Background. Studies in patients with low HDL have suggested that impaired cellular cholesterol efflux is a heritable phenotype increasing atherosclerosis risk. Less is known about the association of macrophage cholesterol efflux with lipid profiles and CAD risk in normolipidemic subjects. We have therefore measured macrophage cholesterol efflux in142 normolipidemic subjects undergoing coronary angiography. Methods. Monocytes isolated from blood samples of patients scheduled for cardiac catheterization were differentiated into macrophages over seven days. Isotopic cholesterol efflux to exogenously added apolipoprotein A-I and HDL2 was measured. Quantitative cholesterol efflux from macrophages was correlated with lipoprotein subclass distribution in plasma from the same individuals measured by NMR-spectroscopy of lipids and with the extent of coronary artery disease seen on coronary angiography. Results. Macrophage cholesterol efflux was positively correlated with particle concentration of smaller HDL and LDL particles but not with total plasma concentrations of HDL or LDL-cholesterol. We observed an inverse relationship between macrophage cholesterol efflux and the concntration of larger and triglyceride rich particles (VLDL, chylomicrons). Subjects with significant stenosis on coronary angiography had lower cholesterol efflux from macrophages compared to individuals without significant stenosis (adjusted p = 0.02). Conclusion. Macrophage cholesterol efflux is inversely correlated with lipoprotein particle size and risk of CAD.
UR - http://www.scopus.com/inward/record.url?scp=65449184505&partnerID=8YFLogxK
U2 - 10.1186/1476-511X-8-14
DO - 10.1186/1476-511X-8-14
M3 - Journal articles
C2 - 19348677
AN - SCOPUS:65449184505
SN - 1476-511X
VL - 8
JO - Lipids in Health and Disease
JF - Lipids in Health and Disease
M1 - 14
ER -