TY - JOUR
T1 - Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney
AU - Ciba, P.
AU - Schicktanz, S.
AU - Anders, E.
AU - Siegl, E.
AU - Stielow, A.
AU - Klink, E.
AU - Kruse, Charli
PY - 2008/2/8
Y1 - 2008/2/8
N2 - In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.
AB - In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.
UR - http://www.scopus.com/inward/record.url?scp=55549136033&partnerID=8YFLogxK
U2 - 10.1007/s10695-007-9196-8
DO - 10.1007/s10695-007-9196-8
M3 - Journal articles
C2 - 18958594
AN - SCOPUS:55549136033
VL - 34
SP - 367
EP - 372
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
SN - 0920-1742
IS - 4
ER -