TY - JOUR
T1 - Local generation of C-reactive protein in diseased coronary artery venous bypass grafts and normal vascular tissue
AU - Jabs, Wolfram J.
AU - Theissing, Elisabeth
AU - Nitschke, Martin
AU - Bechtel, J. F.Matthias
AU - Duchrow, Michael
AU - Mohamed, Salah
AU - Jahrbeck, Bernhard
AU - Sievers, Hans Hinrich
AU - Steinhoff, Jürgen
AU - Bartels, Claus
PY - 2003/9/23
Y1 - 2003/9/23
N2 - Background - Venous coronary artery bypass grafts (CABGs) are prone to accelerated atherosclerosis. In atherosclerotic diseases, serum C-reactive protein (CRP) levels have become an important diagnostic and prognostic marker. The origin of CRP in this setting remains to be elucidated. Methods and Results - Monoclonal anti-CRP identified CRP expression in medial and intimal α-actin-positive smooth muscle cells (SMCs) of diseased CABGs with type V and VI lesions and also of native saphenous veins of atherosclerotic individuals. In addition, patent coronary arteries with type IV and V but not with type I through III lesions exhibited intense SMC staining for CRP. Calcified desobliterates of occluded coronary arteries with end-stage disease did not show SMC staining for CRP and were consistently negative for CRP mRNA, as detected by means of real-time polymerase chain reaction. However, CRP mRNA was expressed in 11 of 15 diseased CABGs and also in 10 of 15 native veins. By contrast, only 3 of 18 internal mammary and 4 of 12 radial arteries with virtually no atherosclerosis were positive for CRP mRNA. Conclusions - CRP is produced by SMCs of atherosclerotic lesions with active disease but not in end-stage plaques. The role of CRP constitutively expressed by normal vascular tissue in vein graft disease has yet to be elucidated.
AB - Background - Venous coronary artery bypass grafts (CABGs) are prone to accelerated atherosclerosis. In atherosclerotic diseases, serum C-reactive protein (CRP) levels have become an important diagnostic and prognostic marker. The origin of CRP in this setting remains to be elucidated. Methods and Results - Monoclonal anti-CRP identified CRP expression in medial and intimal α-actin-positive smooth muscle cells (SMCs) of diseased CABGs with type V and VI lesions and also of native saphenous veins of atherosclerotic individuals. In addition, patent coronary arteries with type IV and V but not with type I through III lesions exhibited intense SMC staining for CRP. Calcified desobliterates of occluded coronary arteries with end-stage disease did not show SMC staining for CRP and were consistently negative for CRP mRNA, as detected by means of real-time polymerase chain reaction. However, CRP mRNA was expressed in 11 of 15 diseased CABGs and also in 10 of 15 native veins. By contrast, only 3 of 18 internal mammary and 4 of 12 radial arteries with virtually no atherosclerosis were positive for CRP mRNA. Conclusions - CRP is produced by SMCs of atherosclerotic lesions with active disease but not in end-stage plaques. The role of CRP constitutively expressed by normal vascular tissue in vein graft disease has yet to be elucidated.
UR - http://www.scopus.com/inward/record.url?scp=0141727734&partnerID=8YFLogxK
U2 - 10.1161/01.CIR.0000092184.43176.91
DO - 10.1161/01.CIR.0000092184.43176.91
M3 - Journal articles
C2 - 12975260
AN - SCOPUS:0141727734
SN - 0009-7322
VL - 108
SP - 1428
EP - 1431
JO - Circulation
JF - Circulation
IS - 12
ER -