Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Dimitrios Spyropoulos; Jan Wenzel

doi:10.1007/978-1-0716-4706-6_11

Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Dimitrios Spyropoulos, Jan Wenzel

Institute of Experimental and Clinical Pharmacology and Toxicology

1 Citation (Scopus)

Abstract

Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

Original language	English
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2956
Pages (from-to)	119-124
Number of pages	6
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-0716-4706-6_11
Publication status	Published - 2025

Funding

Funders	Funder number
H2020 Marie Skłodowska-Curie Actions	813294
Deutsche Forschungsgemeinschaft	WE 6456/1-1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

DFG Research Classification Scheme

2.23-05 Experimental Models for the Understanding of Nervous System Diseases

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10.1007/978-1-0716-4706-6_11

Cite this

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title = "Live Calcium Imaging on Mouse Primary Brain Endothelial Cells",

abstract = "Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.",

author = "Dimitrios Spyropoulos and Jan Wenzel",

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AU - Wenzel, Jan

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

PY - 2025

Y1 - 2025

N2 - Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

AB - Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

UR - https://www.scopus.com/pages/publications/105015239432

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DO - 10.1007/978-1-0716-4706-6_11

M3 - Journal articles

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SN - 1064-3745

VL - 2956

SP - 119

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Abstract

Funding

UN SDGs

Research Areas and Centers

DFG Research Classification Scheme

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