TY - JOUR
T1 - Lithocholic Acid Improves the Survival of Drosophila Melanogaster
AU - Staats, Stefanie
AU - Rimbach, Gerald
AU - Kuenstner, Axel
AU - Graspeuntner, Simon
AU - Rupp, Jan
AU - Busch, Hauke
AU - Sina, Christian
AU - Ipharraguerre, Ignacio R.
AU - Wagner, Anika E.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Scope: Primary bile acids are produced in the liver, whereas secondary bile acids, such as lithocholic acid (LCA), are generated by gut bacteria from primary bile acids that escape the ileal absorption. Besides their well-known function as detergents in lipid digestion, bile acids are important signaling molecules mediating effects on the host's metabolism. Methods and results: Fruit flies (Drosophila melanogaster) are supplemented with 50 μmol L–1 LCA either for 30 days or throughout their lifetime. LCA supplementation results in a significant induction of the mean (+12 days), median (+10 days), and maximum lifespan (+ 11 days) in comparison to untreated control flies. This lifespan extension is accompanied by an induction of spargel (srl), the fly homolog of mammalian PPAR-γ co-activator 1α (PGC1α). In wild-type flies, the administration of antibiotics abrogates both the LCA-mediated lifespan induction as well as the upregulation of srl. Conclusion: It is shown that the secondary bile acid LCA significantly induces the mean, the median, and the maximum survival in D. melanogaster. Our data suggest that besides an upregulation of the PGC1α-homolog srl, unidentified alterations in the structure or metabolism of the gut microbiota contribute to the longevity effect mediated by LCA.
AB - Scope: Primary bile acids are produced in the liver, whereas secondary bile acids, such as lithocholic acid (LCA), are generated by gut bacteria from primary bile acids that escape the ileal absorption. Besides their well-known function as detergents in lipid digestion, bile acids are important signaling molecules mediating effects on the host's metabolism. Methods and results: Fruit flies (Drosophila melanogaster) are supplemented with 50 μmol L–1 LCA either for 30 days or throughout their lifetime. LCA supplementation results in a significant induction of the mean (+12 days), median (+10 days), and maximum lifespan (+ 11 days) in comparison to untreated control flies. This lifespan extension is accompanied by an induction of spargel (srl), the fly homolog of mammalian PPAR-γ co-activator 1α (PGC1α). In wild-type flies, the administration of antibiotics abrogates both the LCA-mediated lifespan induction as well as the upregulation of srl. Conclusion: It is shown that the secondary bile acid LCA significantly induces the mean, the median, and the maximum survival in D. melanogaster. Our data suggest that besides an upregulation of the PGC1α-homolog srl, unidentified alterations in the structure or metabolism of the gut microbiota contribute to the longevity effect mediated by LCA.
UR - http://www.scopus.com/inward/record.url?scp=85052964969&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201800424
DO - 10.1002/mnfr.201800424
M3 - Journal articles
C2 - 30051966
AN - SCOPUS:85052964969
SN - 1613-4125
VL - 62
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 20
M1 - 1800424
ER -