Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues

Axel S. Merseburger; Markus A. Kuczyk; Jürgen Serth; Carsten Bokemeyer; Denise Y. Young; Leon Sun; Roger R. Connelly; David G. McLeod; Fathollah K. Mostofi; Shiv K. Srivastava; Arnulf Stenzl; Judd W. Moul; Isabell A. Sesterhenn

doi:10.3892/or.10.1.223

Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues

Axel S. Merseburger, Markus A. Kuczyk^*, Jürgen Serth, Carsten Bokemeyer, Denise Y. Young, Leon Sun, Roger R. Connelly, David G. McLeod, Fathollah K. Mostofi, Shiv K. Srivastava, Arnulf Stenzl, Judd W. Moul, Isabell A. Sesterhenn

^*Corresponding author for this work

Clinic of Urology

60 Citations (Scopus)

Abstract

Several investigators have reported the correlation of p53 and bcl-2 immunoreactivity with post operative prostate specific antigen (PSA) recurrence. Focal and or clustered expression is typical for these biomarkers. The purpose of this study was to compare the effectiveness of tissue microarrays to detect p53 and bcl-2 overexpression and their prognostic significance. Tissue microarrays (TMA) of 99 patients with mean follow-up of 61 months contained 760 samples from 241 carcinomas, 431 benign glands, and 88 foci of prostatic intraepithelial neoplasia (PIN). Overexpression of p53 was seen in 43.3% of 97 patients, whereas bcl-2 overexpression was noted in 23.7% of 97 patients using TMA technology, compared to 66.0% and 26.9%, respectively in the corresponding radical prostatectomy samples. The tissue microarray technology is a powerful tool to study the multifocal and heterogeneous nature of prostate cancer. However, the prognostic value of p53 and bcl-2 could not be confirmed using this technology in contrast to radical prostatectomy sections. The TMA technique is probably more informative and reliable in evaluating the prognostic value of homogeneously expressed biomarkers.

Original language	English
Journal	Oncology Reports
Volume	10
Issue number	1
Pages (from-to)	223-228
Number of pages	6
ISSN	1021-335X
DOIs	https://doi.org/10.3892/or.10.1.223
Publication status	Published - 01.2003

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Research Area: Luebeck Integrated Oncology Network (LION)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/or.10.1.223

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Merseburger, A. S., Kuczyk, M. A., Serth, J., Bokemeyer, C., Young, D. Y., Sun, L., Connelly, R. R., McLeod, D. G., Mostofi, F. K., Srivastava, S. K., Stenzl, A., Moul, J. W., & Sesterhenn, I. A. (2003). Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues. Oncology Reports, 10(1), 223-228. https://doi.org/10.3892/or.10.1.223

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title = "Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues",

abstract = "Several investigators have reported the correlation of p53 and bcl-2 immunoreactivity with post operative prostate specific antigen (PSA) recurrence. Focal and or clustered expression is typical for these biomarkers. The purpose of this study was to compare the effectiveness of tissue microarrays to detect p53 and bcl-2 overexpression and their prognostic significance. Tissue microarrays (TMA) of 99 patients with mean follow-up of 61 months contained 760 samples from 241 carcinomas, 431 benign glands, and 88 foci of prostatic intraepithelial neoplasia (PIN). Overexpression of p53 was seen in 43.3% of 97 patients, whereas bcl-2 overexpression was noted in 23.7% of 97 patients using TMA technology, compared to 66.0% and 26.9%, respectively in the corresponding radical prostatectomy samples. The tissue microarray technology is a powerful tool to study the multifocal and heterogeneous nature of prostate cancer. However, the prognostic value of p53 and bcl-2 could not be confirmed using this technology in contrast to radical prostatectomy sections. The TMA technique is probably more informative and reliable in evaluating the prognostic value of homogeneously expressed biomarkers.",

author = "Merseburger, {Axel S.} and Kuczyk, {Markus A.} and J{\"u}rgen Serth and Carsten Bokemeyer and Young, {Denise Y.} and Leon Sun and Connelly, {Roger R.} and McLeod, {David G.} and Mostofi, {Fathollah K.} and Srivastava, {Shiv K.} and Arnulf Stenzl and Moul, {Judd W.} and Sesterhenn, {Isabell A.}",

year = "2003",

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doi = "10.3892/or.10.1.223",

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Merseburger, AS, Kuczyk, MA, Serth, J, Bokemeyer, C, Young, DY, Sun, L, Connelly, RR, McLeod, DG, Mostofi, FK, Srivastava, SK, Stenzl, A, Moul, JW & Sesterhenn, IA 2003, 'Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues', Oncology Reports, vol. 10, no. 1, pp. 223-228. https://doi.org/10.3892/or.10.1.223

TY - JOUR

T1 - Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues

AU - Merseburger, Axel S.

AU - Kuczyk, Markus A.

AU - Serth, Jürgen

AU - Bokemeyer, Carsten

AU - Young, Denise Y.

AU - Sun, Leon

AU - Connelly, Roger R.

AU - McLeod, David G.

AU - Mostofi, Fathollah K.

AU - Srivastava, Shiv K.

AU - Stenzl, Arnulf

AU - Moul, Judd W.

AU - Sesterhenn, Isabell A.

PY - 2003/1

Y1 - 2003/1

N2 - Several investigators have reported the correlation of p53 and bcl-2 immunoreactivity with post operative prostate specific antigen (PSA) recurrence. Focal and or clustered expression is typical for these biomarkers. The purpose of this study was to compare the effectiveness of tissue microarrays to detect p53 and bcl-2 overexpression and their prognostic significance. Tissue microarrays (TMA) of 99 patients with mean follow-up of 61 months contained 760 samples from 241 carcinomas, 431 benign glands, and 88 foci of prostatic intraepithelial neoplasia (PIN). Overexpression of p53 was seen in 43.3% of 97 patients, whereas bcl-2 overexpression was noted in 23.7% of 97 patients using TMA technology, compared to 66.0% and 26.9%, respectively in the corresponding radical prostatectomy samples. The tissue microarray technology is a powerful tool to study the multifocal and heterogeneous nature of prostate cancer. However, the prognostic value of p53 and bcl-2 could not be confirmed using this technology in contrast to radical prostatectomy sections. The TMA technique is probably more informative and reliable in evaluating the prognostic value of homogeneously expressed biomarkers.

AB - Several investigators have reported the correlation of p53 and bcl-2 immunoreactivity with post operative prostate specific antigen (PSA) recurrence. Focal and or clustered expression is typical for these biomarkers. The purpose of this study was to compare the effectiveness of tissue microarrays to detect p53 and bcl-2 overexpression and their prognostic significance. Tissue microarrays (TMA) of 99 patients with mean follow-up of 61 months contained 760 samples from 241 carcinomas, 431 benign glands, and 88 foci of prostatic intraepithelial neoplasia (PIN). Overexpression of p53 was seen in 43.3% of 97 patients, whereas bcl-2 overexpression was noted in 23.7% of 97 patients using TMA technology, compared to 66.0% and 26.9%, respectively in the corresponding radical prostatectomy samples. The tissue microarray technology is a powerful tool to study the multifocal and heterogeneous nature of prostate cancer. However, the prognostic value of p53 and bcl-2 could not be confirmed using this technology in contrast to radical prostatectomy sections. The TMA technique is probably more informative and reliable in evaluating the prognostic value of homogeneously expressed biomarkers.

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AN - SCOPUS:0038340752

SN - 1021-335X

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EP - 228

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JF - Oncology Reports

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ER -

Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues

Abstract

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