Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

Sazgar Burhan; Madita Göb; Mario Pieper; Tjalfe Laedtke; Thorge Grahl; Michael Münter; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Peter König; Robert Huber

doi:10.1117/12.3046216

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

Sazgar Burhan, Madita Göb, Mario Pieper, Tjalfe Laedtke, Thorge Grahl, Michael Münter, Hinnerk Schulz-Hildebrandt, Gereon Hüttmann, Peter König, Robert Huber

Abstract

We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm². Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

Original language	English
Title of host publication	Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX
Editors	Rainer A. Leitgeb, Yoshiaki Yasuno
Number of pages	1
Volume	13305
Publisher	SPIE
Publication date	03.2025
Pages	1330502
DOIs	https://doi.org/10.1117/12.3046216
Publication status	Published - 03.2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.3046216

https://doi.org/10.1117/12.3046216

Cite this

Burhan, S., Göb, M., Pieper, M., Laedtke, T., Grahl, T., Münter, M., Schulz-Hildebrandt, H., Hüttmann, G., König, P., & Huber, R. (2025). Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. In R. A. Leitgeb, & Y. Yasuno (Eds.), Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX (Vol. 13305, pp. 1330502). SPIE. https://doi.org/10.1117/12.3046216

@inproceedings{cbdee7dc33114844bd13531a1172e423,

title = "Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue",

abstract = "We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system{\textquoteright}s ability to visualize cellular tissue structures.",

author = "Sazgar Burhan and Madita G{\"o}b and Mario Pieper and Tjalfe Laedtke and Thorge Grahl and Michael M{\"u}nter and Hinnerk Schulz-Hildebrandt and Gereon H{\"u}ttmann and Peter K{\"o}nig and Robert Huber",

year = "2025",

month = mar,

doi = "10.1117/12.3046216",

language = "English",

volume = "13305",

pages = "1330502",

editor = "Leitgeb, {Rainer A.} and Yoshiaki Yasuno",

booktitle = "Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX",

publisher = "SPIE",

}

Burhan, S, Göb, M , Pieper, M, Laedtke, T, Grahl, T, Münter, M, Schulz-Hildebrandt, H, Hüttmann, G , König, P & Huber, R 2025, Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. in RA Leitgeb & Y Yasuno (eds), Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX. vol. 13305, SPIE, pp. 1330502. https://doi.org/10.1117/12.3046216

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. / Burhan, Sazgar; Göb, Madita ; Pieper, Mario et al.
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX. ed. / Rainer A. Leitgeb; Yoshiaki Yasuno. Vol. 13305 SPIE, 2025. p. 1330502.

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

TY - GEN

T1 - Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

AU - Burhan, Sazgar

AU - Göb, Madita

AU - Pieper, Mario

AU - Laedtke, Tjalfe

AU - Grahl, Thorge

AU - Münter, Michael

AU - Schulz-Hildebrandt, Hinnerk

AU - Hüttmann, Gereon

AU - König, Peter

AU - Huber, Robert

PY - 2025/3

Y1 - 2025/3

N2 - We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

AB - We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

U2 - 10.1117/12.3046216

DO - 10.1117/12.3046216

M3 - Conference contribution

VL - 13305

SP - 1330502

BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX

A2 - Leitgeb, Rainer A.

A2 - Yasuno, Yoshiaki

PB - SPIE

ER -

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this