Lanthanide-chelating carbohydrate conjugates are useful tools to characterize carbohydrate conformation in solution and sensitive sensors to detect carbohydrate-protein interactions

Ángeles Canales, Álvaro Mallagaray, M. Álvaro Berbís, Armando Navarro-Vázquez, Gema Domínguez, F. Javier Cañada, Sabine André, Hans Joachim Gabius, Javier Pérez-Castells, Jesús Jiménez-Barbero*

*Corresponding author for this work
36 Citations (Scopus)

Abstract

The increasing interest in the functional versatility of glycan epitopes in cellular glycoconjugates calls for developing sensitive methods to define carbohydrate conformation in solution and to characterize protein-carbohydrate interactions. Measurements of pseudocontact shifts in the presence of a paramagnetic cation can provide such information. In this work, the energetically privileged conformation of a disaccharide (lactose as test case) was experimentally inferred by using a synthetic carbohydrate conjugate bearing a lanthanide binding tag. In addition, the binding of lactose to a biomedically relevant receptor (the human adhesion/growth-regulatory lectin galectin-3) and its consequences in structural terms were defined, using Dy3+, Tb3+, and Tm3+. The described approach, complementing the previously tested protein tagging as a way to exploit paramagnetism, enables to detect binding, even weak interactions, and to characterize in detail topological aspects useful for physiological ligands and mimetics in drug design.

Original languageEnglish
JournalJournal of the American Chemical Society
Volume136
Issue number22
Pages (from-to)8011-8017
Number of pages7
ISSN0002-7863
DOIs
Publication statusPublished - 04.06.2014

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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