TY - JOUR
T1 - Jinxed
T2 - just in time crystallization for easy structure determination of biological macromolecules
AU - Henkel, Alessandra
AU - Galchenkova, Marina
AU - Maracke, Julia
AU - Yefanov, Oleksandr
AU - Klopprogge, Bjarne
AU - Hakanpää, Johanna
AU - Mesters, Jeroen R
AU - Chapman, Henry N
AU - Oberthuer, Dominik
N1 - Publisher Copyright:
© 2023 International Union of Crystallography. All rights reserved.
PY - 2023/9
Y1 - 2023/9
N2 - Macromolecular crystallography is a well established method in the field of structural biology and has led to the majority of known protein structures to date. After focusing on static structures, the method is now under development towards the investigation of protein dynamics through time-resolved methods. These experiments often require multiple handling steps of the sensitive protein crystals, e.g. for ligand-soaking and cryo-protection. These handling steps can cause significant crystal damage, and hence reduce data quality. Furthermore, in time-resolved experiments based on serial crystallography, which use micrometre-sized crystals for short diffusion times of ligands, certain crystal morphologies with small solvent channels can prevent sufficient ligand diffusion. Described here is a method that combines protein crystallization and data collection in a novel one-step process. Corresponding experiments were successfully performed as a proof-of-principle using hen egg-white lysozyme and crystallization times of only a few seconds. This method, called JINXED (Just IN time Crystallization for Easy structure Determination), promises high-quality data due to the avoidance of crystal handling and has the potential to enable time-resolved experiments with crystals containing small solvent channels by adding potential ligands to the crystallization buffer, simulating traditional co-crystallization approaches.
AB - Macromolecular crystallography is a well established method in the field of structural biology and has led to the majority of known protein structures to date. After focusing on static structures, the method is now under development towards the investigation of protein dynamics through time-resolved methods. These experiments often require multiple handling steps of the sensitive protein crystals, e.g. for ligand-soaking and cryo-protection. These handling steps can cause significant crystal damage, and hence reduce data quality. Furthermore, in time-resolved experiments based on serial crystallography, which use micrometre-sized crystals for short diffusion times of ligands, certain crystal morphologies with small solvent channels can prevent sufficient ligand diffusion. Described here is a method that combines protein crystallization and data collection in a novel one-step process. Corresponding experiments were successfully performed as a proof-of-principle using hen egg-white lysozyme and crystallization times of only a few seconds. This method, called JINXED (Just IN time Crystallization for Easy structure Determination), promises high-quality data due to the avoidance of crystal handling and has the potential to enable time-resolved experiments with crystals containing small solvent channels by adding potential ligands to the crystallization buffer, simulating traditional co-crystallization approaches.
UR - http://www.scopus.com/inward/record.url?scp=85159577658&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/03bef051-6133-3779-9819-b56bd6505f86/
U2 - 10.1107/S2052252523001653
DO - 10.1107/S2052252523001653
M3 - Journal articles
C2 - 36892542
SN - 2052-2525
VL - 10
SP - 253
EP - 260
JO - IUCrJ
JF - IUCrJ
IS - Pt 3
ER -