Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

Julian Christopher Assmann; Kristin Müller; Jan Wenzel; Thomas Walther; Josefine Brands; Peter Thornton; Markus Schwaninger; Stuart M Allan

doi:10.21769/BioProtoc.2294

Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

Julian Christopher Assmann, Kristin Müller, Jan Wenzel, Thomas Walther, Josefine Brands, Peter Thornton, Markus Schwaninger, Stuart M Allan

Institute of Experimental and Clinical Pharmacology and Toxicology

Abstract

Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, outgrowing endothelial cells are selected by puromycin treatment and grown to confluence within one week.

Original language	English
Journal	Bio-protocol
Volume	7
Issue number	10
Pages (from-to)	e2294
Number of pages	1
ISSN	2331-8325
DOIs	https://doi.org/10.21769/BioProtoc.2294
Publication status	Published - 20.05.2017

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21769/BioProtoc.2294

Cite this

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title = "Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice",

abstract = "Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, outgrowing endothelial cells are selected by puromycin treatment and grown to confluence within one week.",

author = "Assmann, {Julian Christopher} and Kristin M{\"u}ller and Jan Wenzel and Thomas Walther and Josefine Brands and Peter Thornton and Markus Schwaninger and Allan, {Stuart M}",

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T1 - Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

AU - Assmann, Julian Christopher

AU - Müller, Kristin

AU - Wenzel, Jan

AU - Walther, Thomas

AU - Brands, Josefine

AU - Thornton, Peter

AU - Schwaninger, Markus

AU - Allan, Stuart M

PY - 2017/5/20

Y1 - 2017/5/20

N2 - Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, outgrowing endothelial cells are selected by puromycin treatment and grown to confluence within one week.

AB - Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, outgrowing endothelial cells are selected by puromycin treatment and grown to confluence within one week.

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464392/

U2 - 10.21769/BioProtoc.2294

DO - 10.21769/BioProtoc.2294

M3 - Journal articles

SN - 2331-8325

VL - 7

SP - e2294

JO - Bio-protocol

JF - Bio-protocol

IS - 10

ER -

Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

Abstract

Research Areas and Centers

UN SDGs

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