Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Christine Zühlke; Rasmus Kiehl; Antje Johannsmeyer; Karl Heinz Grzeschik; Eberhard Schwinger

doi:10.3109/10425179909033929

Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Christine Zühlke^*, Rasmus Kiehl, Antje Johannsmeyer, Karl Heinz Grzeschik, Eberhard Schwinger

^*Corresponding author for this work

Institute of Human Genetics

University of Marburg

5 Citations (Scopus)

Abstract

Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

Original language	English
Journal	Mitochondrial DNA
Volume	10
Issue number	1
Pages (from-to)	1-6
Number of pages	6
ISSN	1940-1736
DOIs	https://doi.org/10.3109/10425179909033929
Publication status	Published - 1999

Funding

This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Research Area: Medical Genetics

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Cite this

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title = "Isolation and characterization of novel CAG repeat containing genes expressed in human brain",

abstract = "Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 \% of chromosomes) and 7 triplets (20 \% of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.",

author = "Christine Z{\"u}hlke and Rasmus Kiehl and Antje Johannsmeyer and Grzeschik, \{Karl Heinz\} and Eberhard Schwinger",

note = "Funding Information: This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.",

year = "1999",

doi = "10.3109/10425179909033929",

language = "English",

volume = "10",

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T1 - Isolation and characterization of novel CAG repeat containing genes expressed in human brain

AU - Zühlke, Christine

AU - Kiehl, Rasmus

AU - Johannsmeyer, Antje

AU - Grzeschik, Karl Heinz

AU - Schwinger, Eberhard

N1 - Funding Information: This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.

PY - 1999

Y1 - 1999

N2 - Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

AB - Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

UR - https://www.scopus.com/pages/publications/0344223299

U2 - 10.3109/10425179909033929

DO - 10.3109/10425179909033929

M3 - Journal articles

C2 - 10565538

AN - SCOPUS:0344223299

SN - 1940-1736

VL - 10

SP - 1

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JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 1

ER -

Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Abstract

Funding

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Research Areas and Centers

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