Investigations of iron uptake in Halobacterium salinarum

D. Hubmacher; B. F. Matzanke; S. Anemüller

doi:10.1042/BST0300710

Investigations of iron uptake in Halobacterium salinarum

D. Hubmacher, B. F. Matzanke, S. Anemüller^*

^*Corresponding author for this work

6 Citations (Scopus)

Abstract

The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of ⁵⁵Fe and [¹⁴C]citrate gave evidence only for the accumulation of iron. Two additional membrane proteins could be detected in iron-starved cells, one in iron-repleted membranes and one that is up-regulated there. Respiratory rates of iron-starved membranes after the addition of succinate and NADH differed considerably from iron-repleted ones. Furthermore, both types of membrane exhibited different degrees of inhibition by cyanide.

Original language	English
Journal	Biochemical Society Transactions
Volume	30
Issue number	4
Pages (from-to)	710-712
Number of pages	3
ISSN	0300-5127
DOIs	https://doi.org/10.1042/BST0300710
Publication status	Published - 08.2002

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Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1042/BST0300710

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title = "Investigations of iron uptake in Halobacterium salinarum",

abstract = "The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of 55Fe and [14C]citrate gave evidence only for the accumulation of iron. Two additional membrane proteins could be detected in iron-starved cells, one in iron-repleted membranes and one that is up-regulated there. Respiratory rates of iron-starved membranes after the addition of succinate and NADH differed considerably from iron-repleted ones. Furthermore, both types of membrane exhibited different degrees of inhibition by cyanide.",

author = "D. Hubmacher and Matzanke, \{B. F.\} and S. Anem{\"u}ller",

year = "2002",

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doi = "10.1042/BST0300710",

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T1 - Investigations of iron uptake in Halobacterium salinarum

AU - Hubmacher, D.

AU - Matzanke, B. F.

AU - Anemüller, S.

PY - 2002/8

Y1 - 2002/8

N2 - The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of 55Fe and [14C]citrate gave evidence only for the accumulation of iron. Two additional membrane proteins could be detected in iron-starved cells, one in iron-repleted membranes and one that is up-regulated there. Respiratory rates of iron-starved membranes after the addition of succinate and NADH differed considerably from iron-repleted ones. Furthermore, both types of membrane exhibited different degrees of inhibition by cyanide.

AB - The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of 55Fe and [14C]citrate gave evidence only for the accumulation of iron. Two additional membrane proteins could be detected in iron-starved cells, one in iron-repleted membranes and one that is up-regulated there. Respiratory rates of iron-starved membranes after the addition of succinate and NADH differed considerably from iron-repleted ones. Furthermore, both types of membrane exhibited different degrees of inhibition by cyanide.

UR - https://www.scopus.com/pages/publications/0036669922

U2 - 10.1042/BST0300710

DO - 10.1042/BST0300710

M3 - Journal articles

C2 - 12196172

AN - SCOPUS:0036669922

SN - 0300-5127

VL - 30

SP - 710

EP - 712

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - 4

ER -

Investigations of iron uptake in Halobacterium salinarum

Abstract

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