Investigation of laser-induced cell lysis using time-resolved imaging

Kaustubh R. Rau; Arnold Guerra; Alfred Vogel; Vasan Venugopalan

doi:10.1063/1.1705728

Investigation of laser-induced cell lysis using time-resolved imaging

Kaustubh R. Rau, Arnold Guerra, Alfred Vogel, Vasan Venugopalan^*

^*Corresponding author for this work

Institute of Biomedical Optics

University of California, Irvine

101 Citations (Scopus)

Abstract

The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.

Original language	English
Journal	Applied Physics Letters
Volume	84
Issue number	15
Pages (from-to)	2940-2942
Number of pages	3
ISSN	0003-6951
DOIs	https://doi.org/10.1063/1.1705728
Publication status	Published - 12.04.2004

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Academic Focus: Biomedical Engineering

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10.1063/1.1705728

Cite this

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title = "Investigation of laser-induced cell lysis using time-resolved imaging",

abstract = "The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.",

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N2 - The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.

AB - The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.

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Investigation of laser-induced cell lysis using time-resolved imaging

Abstract

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