Insights into neuronal cell metabolism using NMR spectroscopy: Uridyl diphosphate N-acetyl-glucosamine as a unique metabolic marker

Anika Gallinger; Thorsten Biet; Luc Pellerin; Thomas Peters

doi:10.1002/anie.201104836

Insights into neuronal cell metabolism using NMR spectroscopy: Uridyl diphosphate N-acetyl-glucosamine as a unique metabolic marker

Anika Gallinger, Thorsten Biet, Luc Pellerin, Thomas Peters^*

^*Corresponding author for this work

Institute of Chemistry and Metabolomics

University of Lausanne

6 Citations (Scopus)

Abstract

Making the switch: Compounds 1 and 2 are used as metabolic markers for NMR detection. When neuronal cells switch to a glycolytic state, an uneven distribution of ¹³C in the N-acetyl group results, thus giving a mixture of the metabolites 1 and 2. It is therefore possible to monitor flux through different metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, and the hexosamine biosynthetic pathway, using a single molecule.

Original language	English
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	49
Pages (from-to)	11672-11674
Number of pages	3
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201104836
Publication status	Published - 02.12.2011

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201104836

Cite this

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title = "Insights into neuronal cell metabolism using NMR spectroscopy: Uridyl diphosphate N-acetyl-glucosamine as a unique metabolic marker",

abstract = "Making the switch: Compounds 1 and 2 are used as metabolic markers for NMR detection. When neuronal cells switch to a glycolytic state, an uneven distribution of 13C in the N-acetyl group results, thus giving a mixture of the metabolites 1 and 2. It is therefore possible to monitor flux through different metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, and the hexosamine biosynthetic pathway, using a single molecule.",

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AU - Peters, Thomas

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Insights into neuronal cell metabolism using NMR spectroscopy: Uridyl diphosphate N-acetyl-glucosamine as a unique metabolic marker

Abstract

Research Areas and Centers

UN SDGs

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CRU 126, Subproject: NMR studies on the molecular basis of the pull principle in the astrocyte-neuron shuttle

Cite this

Insights into neuronal cell metabolism using NMR spectroscopy: Uridyl diphosphate N-acetyl-glucosamine as a unique metabolic marker

Abstract

Research Areas and Centers

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

CRU 126, Subproject: NMR studies on the molecular basis of the pull principle in the astrocyte-neuron shuttle

Cite this