Insight into the mechanism of a peptide inhibitor of HIV reverse transcriptase dimerization

Julien Depollier, Marie Laure Hourdou, Gudrun Aldrian-Herrada, Paul Rothwell, Tobias Restle, Gilles Divita*

*Corresponding author for this work
    24 Citations (Scopus)

    Abstract

    The biologically active forms of human immunodeficiency viruses type 1 and 2 reverse transcriptase (RT) found in infectious virions are heterodimers. We have previously shown that the dimeric nature of reverse transcriptase represents an important target for the design of a new class of antiviral agents and have designed a short peptide (Pep-7) derived from the tryptophan-rich motif of the connection subdomain that blocks dimerization of reverse transcriptase in vitro and abolishes viral infection. In the present work, we have investigated the mechanism through which this peptide inhibits RT dimerization and consequently viral propagation. We demonstrate that Pep-7 interacts preferentially with the p51 subunit within the heterodimeric reverse transcriptase, which destabilizes reverse transcriptase dimer conformation, thereby triggering dissociation. We have identified two residues Trp 24 and Phe61, located on the fingers subdomain of p51, required for Pep-7 binding. Selective mutation of these residues on p51 to a glycine dramatically alters the stability of the RT-heterodimer suggesting that the fingers subdomain of p51 is also involved in stabilization of reverse transcriptase. We propose that the binding site of Pep-7 is located in a cleft between the fingers and the connection subdomains of p51 that contains the two highly conserved residues Phe61 and Trp24.

    Original languageEnglish
    JournalBiochemistry
    Volume44
    Issue number6
    Pages (from-to)1909-1918
    Number of pages10
    ISSN0006-2960
    DOIs
    Publication statusPublished - 15.02.2005

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