Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane

Swaantje Peters; Eva Reinthal; Petra Blitgen-Heinecke; Karl-Ulrich Bartz-Schmidt; Ulrich Schraermeyer

doi:10.1159/000090268

Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane

Swaantje Peters, Eva Reinthal, Petra Blitgen-Heinecke, Karl-Ulrich Bartz-Schmidt, Ulrich Schraermeyer

University of Tubingen

Abstract

PURPOSE: To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM).

METHODS: Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy.

RESULTS: Intercellular spaces between the RPE cells and BM were enlarged. Residual material from phagosomes was released into these enlarged spaces. Debris accumulated within BM and encircled choriocapillaris endothelial cells.

CONCLUSION: There is a release of undegraded phagocytic material (rod outer segments) into the extracellular space between BM and RPE cells, following inhibition of lysosomal degradation. Electron-dense deposits in BM and choriocapillaris may lead to reduced oxygen and nutrition flow.

Original language	English
Journal	Ophthalmic Research
Volume	38
Issue number	2
Pages (from-to)	83-8
Number of pages	6
ISSN	0030-3747
DOIs	https://doi.org/10.1159/000090268
Publication status	Published - 2006
Externally published	Yes

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title = "Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane",

abstract = "PURPOSE: To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM).METHODS: Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy.RESULTS: Intercellular spaces between the RPE cells and BM were enlarged. Residual material from phagosomes was released into these enlarged spaces. Debris accumulated within BM and encircled choriocapillaris endothelial cells.CONCLUSION: There is a release of undegraded phagocytic material (rod outer segments) into the extracellular space between BM and RPE cells, following inhibition of lysosomal degradation. Electron-dense deposits in BM and choriocapillaris may lead to reduced oxygen and nutrition flow.",

author = "Swaantje Peters and Eva Reinthal and Petra Blitgen-Heinecke and Karl-Ulrich Bartz-Schmidt and Ulrich Schraermeyer",

year = "2006",

doi = "10.1159/000090268",

language = "English",

volume = "38",

pages = "83--8",

journal = "Ophthalmic Research",

issn = "0030-3747",

number = "2",

}

TY - JOUR

T1 - Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane

AU - Peters, Swaantje

AU - Reinthal, Eva

AU - Blitgen-Heinecke, Petra

AU - Bartz-Schmidt, Karl-Ulrich

AU - Schraermeyer, Ulrich

PY - 2006

Y1 - 2006

N2 - PURPOSE: To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM).METHODS: Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy.RESULTS: Intercellular spaces between the RPE cells and BM were enlarged. Residual material from phagosomes was released into these enlarged spaces. Debris accumulated within BM and encircled choriocapillaris endothelial cells.CONCLUSION: There is a release of undegraded phagocytic material (rod outer segments) into the extracellular space between BM and RPE cells, following inhibition of lysosomal degradation. Electron-dense deposits in BM and choriocapillaris may lead to reduced oxygen and nutrition flow.

AB - PURPOSE: To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM).METHODS: Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy.RESULTS: Intercellular spaces between the RPE cells and BM were enlarged. Residual material from phagosomes was released into these enlarged spaces. Debris accumulated within BM and encircled choriocapillaris endothelial cells.CONCLUSION: There is a release of undegraded phagocytic material (rod outer segments) into the extracellular space between BM and RPE cells, following inhibition of lysosomal degradation. Electron-dense deposits in BM and choriocapillaris may lead to reduced oxygen and nutrition flow.

U2 - 10.1159/000090268

DO - 10.1159/000090268

M3 - Journal articles

C2 - 16352920

SN - 0030-3747

VL - 38

SP - 83

EP - 88

JO - Ophthalmic Research

JF - Ophthalmic Research

IS - 2

ER -

Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane

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