Abstract
Surfactant has been shown to influence a variety of immune functions. However, for in vitro studies most investigators used a single surfactant concentration often far below what has to be expected in bronchoalveolar fluid following surfactant replacement therapy. We studied the chemotactic activity and the oxygen metabolite release of human polymorphonuclear leukocytes (PMN) following incubation with Curosurf®, a porcine modified natural surfactant preparation, at concentrations ranging from 1 to 16 mg/ml. In the presence of 1% bovine serum albumin, surfactant at 1 and 4 mg/ml enhanced anaphylatoxin C5a-related chemotaxis, whereas a higher dose of 16 mg/ml was inhibitory. Furthermore, Curosurf® itself demonstrated a concentration-dependent chemotactic effect. Oxygen metabolite release, as measured by nitroblue tetrazolium reduction, was significantly diminished at surfactant concentrations of 8 and 16 mg/ml. This effect was most pronounced when group B streptococci at concentrations 5 × 109 CFU/ml were applied for PMN stimulation. We conclude that the effects of surfactant on PMN immune functions are not only concentration-dependent but also influenced by the degree of PMN stimulation.
| Original language | English |
|---|---|
| Journal | Immunobiology |
| Volume | 205 |
| Issue number | 3 |
| Pages (from-to) | 290-302 |
| Number of pages | 13 |
| ISSN | 0171-2985 |
| DOIs | |
| Publication status | Published - 2002 |
Funding
The authors wish to thank Gabi Walter and Ines Heine for excellent technical support. The authors are grateful to Prof. Bengt Robertson, Research Unit for Experimental Perinatal Pathology, Karolinska Institute, Stockholm, Sweden for critical discussion of the manuscript. This study was supported by the Deutsche Forschungsgemeinschaft (DFG He 2072/2-2). Surfactant (Curosurf®) was a generous gift from Serono/Nycomed Pharma, Munich, Germany.
Research Areas and Centers
- Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)
