TY - JOUR
T1 - Influence of modified natural and synthetic surfactant preparations on bacterial killing by polymorphonuclear leucocytes
AU - Rauprich, Petra
AU - Walter, Gabriele
AU - Jarstrand, Connie
AU - Robertson, Bengt
AU - Herting, Egbert
N1 - Funding Information:
The authors declare no competing financial interests. The surfactant preparations for the studies were generously supplied by the respective manufacturers (see Methods). E.H. has been involved in clinical studies testing Alveofact ® (Boehringer Ltd), Curosurf ® (Chiesi Pharmceuticals) and Survanta ® (Ross Laboratories) and has therefore received financial support and/or travel grants from these companies.
Funding Information:
The study was supported by the German Research Council (Deutsche Forschungsgemeinschaft He 2072/2–2) and a collaborative project of the German Academic Exchange Service and the Swedish Institute (313/S-PPP).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2004
Y1 - 2004
N2 - In addition to its biophysical functions, surfactant plays an important role in pulmonary host defense. In this investigation we studied the influence of various commercially available surfactants on the phagocytosis of bacteria that are common pathogens in the neonatal period. Group B streptococci (GBS), Escherichia coli and Staphylococcus aureus were cultured with isolated human polymorphonuclear leucocytes (PMN) and non-specific serum in the presence or absence of different modified natural (Curosurf®, Alveofact®, Survanta® or totally synthetic, protein-free surfactant preparations (Exosurf®, Pumactant®). Prior to and after 30 and 60 min of incubation with PMN at different surfactant concentrations (1, 10 or 20 mg/ml), the number of viable bacteria was determined by colony counting. Killing of S. aureus by PMN was not influenced by any of the surfactants. Alveofact® and Curosurf® had no significant negative impact on phagocytosis. At 20 mg/ml, Curosurf® even reduced the number of viable E. coli. Survanta® at 10 and 20 mg/ml and Exosurf® at all concentrations impaired the killing of non-encapsulated GBS and E. coli. Pumactant® at 1-20 mg/ml interfered with the phagocytosis of E. coli. In further experiments we demonstrated that Curosurf® did not interfere with the phagocytosis of an encapsulated GBS-strain opsonised by a specific antiserum either. In additional experiments we analysed the influence of the different surfactants on the release of reactive oxygen metabolite by PMN and found that the changes in nitroblue tetrazolium. reduction did not necessarily correlate with the findings of the studies on killing. In conclusion, we found that killing by PMN was influenced by the bacterial species and the composition and concentration of the different surfactant preparations. The strongest impairment in phagocytic function of PMN was observed with the protein-free synthetic surfactant Exosurf®, a phospholipid preparation that contains the alcohols hexadecanol and tyloxapol as spreading agents.
AB - In addition to its biophysical functions, surfactant plays an important role in pulmonary host defense. In this investigation we studied the influence of various commercially available surfactants on the phagocytosis of bacteria that are common pathogens in the neonatal period. Group B streptococci (GBS), Escherichia coli and Staphylococcus aureus were cultured with isolated human polymorphonuclear leucocytes (PMN) and non-specific serum in the presence or absence of different modified natural (Curosurf®, Alveofact®, Survanta® or totally synthetic, protein-free surfactant preparations (Exosurf®, Pumactant®). Prior to and after 30 and 60 min of incubation with PMN at different surfactant concentrations (1, 10 or 20 mg/ml), the number of viable bacteria was determined by colony counting. Killing of S. aureus by PMN was not influenced by any of the surfactants. Alveofact® and Curosurf® had no significant negative impact on phagocytosis. At 20 mg/ml, Curosurf® even reduced the number of viable E. coli. Survanta® at 10 and 20 mg/ml and Exosurf® at all concentrations impaired the killing of non-encapsulated GBS and E. coli. Pumactant® at 1-20 mg/ml interfered with the phagocytosis of E. coli. In further experiments we demonstrated that Curosurf® did not interfere with the phagocytosis of an encapsulated GBS-strain opsonised by a specific antiserum either. In additional experiments we analysed the influence of the different surfactants on the release of reactive oxygen metabolite by PMN and found that the changes in nitroblue tetrazolium. reduction did not necessarily correlate with the findings of the studies on killing. In conclusion, we found that killing by PMN was influenced by the bacterial species and the composition and concentration of the different surfactant preparations. The strongest impairment in phagocytic function of PMN was observed with the protein-free synthetic surfactant Exosurf®, a phospholipid preparation that contains the alcohols hexadecanol and tyloxapol as spreading agents.
UR - http://www.scopus.com/inward/record.url?scp=11244261268&partnerID=8YFLogxK
U2 - 10.1016/j.imbio.2004.09.002
DO - 10.1016/j.imbio.2004.09.002
M3 - Journal articles
C2 - 15638129
AN - SCOPUS:11244261268
VL - 209
SP - 609
EP - 617
JO - Immunobiology
JF - Immunobiology
SN - 0171-2985
IS - 8
ER -
