TY - JOUR
T1 - Induction of the cytokine TWEAK and its receptor Fn14 in ischemic stroke
AU - Inta, Ioana
AU - Frauenknecht, Katrin
AU - Dörr, Henrike
AU - Kohlhof, Patricia
AU - Rabsilber, Tanja
AU - Auffarth, Gerd U.
AU - Burkly, Linda
AU - Mittelbronn, Michel
AU - Hahm, Kyungmin
AU - Sommer, Clemens
AU - Schwaninger, Markus
PY - 2008/12/15
Y1 - 2008/12/15
N2 - Stroke outcome is determined by delayed neuronal cell death and edema formation. TWEAK, a cytokine of the TNF superfamily, and its membrane receptor Fn14 promote ischemia-induced neuronal apoptosis and leakage of the blood-brain barrier. Both TWEAK and Fn14 are upregulated in experimental stroke models. In this study, we investigated whether TWEAK and Fn14 are upregulated in stroke patients. We measured serum concentrations of TWEAK in stroke patients and matched control subjects by ELISA. Expression of Fn14 in the brain was evaluated by real-time RT-PCR and immunohistochemistry. TWEAK serum concentrations were elevated in stroke patients. In autopsy samples, we found elevated mRNA levels of the receptor Fn14 and a trend towards higher TWEAK mRNA levels. In the infarcted and peri-infarct tissue immunostaining for Fn14 was enhanced. These data show that the cytokine TWEAK and its membrane receptor Fn14 are upregulated in stroke and suggest that they contribute to stroke outcome.
AB - Stroke outcome is determined by delayed neuronal cell death and edema formation. TWEAK, a cytokine of the TNF superfamily, and its membrane receptor Fn14 promote ischemia-induced neuronal apoptosis and leakage of the blood-brain barrier. Both TWEAK and Fn14 are upregulated in experimental stroke models. In this study, we investigated whether TWEAK and Fn14 are upregulated in stroke patients. We measured serum concentrations of TWEAK in stroke patients and matched control subjects by ELISA. Expression of Fn14 in the brain was evaluated by real-time RT-PCR and immunohistochemistry. TWEAK serum concentrations were elevated in stroke patients. In autopsy samples, we found elevated mRNA levels of the receptor Fn14 and a trend towards higher TWEAK mRNA levels. In the infarcted and peri-infarct tissue immunostaining for Fn14 was enhanced. These data show that the cytokine TWEAK and its membrane receptor Fn14 are upregulated in stroke and suggest that they contribute to stroke outcome.
UR - http://www.scopus.com/inward/record.url?scp=55649111573&partnerID=8YFLogxK
U2 - 10.1016/j.jns.2008.08.005
DO - 10.1016/j.jns.2008.08.005
M3 - Journal articles
C2 - 18793781
AN - SCOPUS:55649111573
SN - 0022-510X
VL - 275
SP - 117
EP - 120
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
IS - 1-2
ER -
