Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid

J. Jankásková; O. N. Horváth; R. Varga; P. Arenberger; E. Schmidt; T. Ruzicka; M. Sárdy

doi:10.1111/ced.13371

Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid

J. Jankásková, O. N. Horváth, R. Varga, P. Arenberger, E. Schmidt, T. Ruzicka, M. Sárdy^*

^*Corresponding author for this work

Clinic of Dermatology, Allergology and Venerology

11 Citations (Scopus)

Abstract

Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG₁, IgG₃ and IgG₄ autoantibodies. Results: The sensitivities of IIF in detecting IgG₁, IgG₃, IgG₄ and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG₁ and IgG₄) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.

Original language	English
Journal	Clinical and Experimental Dermatology
Volume	43
Issue number	3
Pages (from-to)	248-253
Number of pages	6
ISSN	0307-6938
DOIs	https://doi.org/10.1111/ced.13371
Publication status	Published - 04.2018

Funding

We thank Mr I. Anić, Ms A. Hess and Ms K. Woischwill (Department of Dermatology and Allergology, Ludwig Maximilian University, Munich, Germany) for performing part of the serological and DIF examinations. This study was supported by the Wissenschaftliches Heraus-geberkollegium der Mu€nchener Medizinischen Wochen-schrift e.V.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1111/ced.13371

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@article{35d152bbd6da4f28be2f7d8922ef14d2,

title = "Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid",

abstract = "Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80\%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3\%, 18.8\%, 32.8\% and 48.4\%, respectively. Specificities were > 97\%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.",

author = "J. Jank{\'a}skov{\'a} and Horv{\'a}th, \{O. N.\} and R. Varga and P. Arenberger and E. Schmidt and T. Ruzicka and M. S{\'a}rdy",

year = "2018",

month = apr,

doi = "10.1111/ced.13371",

language = "English",

volume = "43",

pages = "248--253",

journal = "Clinical and Experimental Dermatology",

issn = "0307-6938",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid

AU - Jankásková, J.

AU - Horváth, O. N.

AU - Varga, R.

AU - Arenberger, P.

AU - Schmidt, E.

AU - Ruzicka, T.

AU - Sárdy, M.

PY - 2018/4

Y1 - 2018/4

N2 - Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.

AB - Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.

UR - https://www.scopus.com/pages/publications/85043708992

U2 - 10.1111/ced.13371

DO - 10.1111/ced.13371

M3 - Journal articles

C2 - 29333745

AN - SCOPUS:85043708992

SN - 0307-6938

VL - 43

SP - 248

EP - 253

JO - Clinical and Experimental Dermatology

JF - Clinical and Experimental Dermatology

IS - 3

ER -

Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid

Abstract

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Research Areas and Centers

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