TY - JOUR
T1 - Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid
AU - Jankásková, J.
AU - Horváth, O. N.
AU - Varga, R.
AU - Arenberger, P.
AU - Schmidt, E.
AU - Ruzicka, T.
AU - Sárdy, M.
PY - 2018/4
Y1 - 2018/4
N2 - Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.
AB - Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.
UR - http://www.scopus.com/inward/record.url?scp=85043708992&partnerID=8YFLogxK
U2 - 10.1111/ced.13371
DO - 10.1111/ced.13371
M3 - Journal articles
C2 - 29333745
AN - SCOPUS:85043708992
SN - 0307-6938
VL - 43
SP - 248
EP - 253
JO - Clinical and Experimental Dermatology
JF - Clinical and Experimental Dermatology
IS - 3
ER -