Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid

J. Jankásková, O. N. Horváth, R. Varga, P. Arenberger, E. Schmidt, T. Ruzicka, M. Sárdy*

*Corresponding author for this work
6 Citations (Scopus)

Abstract

Background: Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60–80%) may be partly due to insufficient detection of minor IgG subclasses. Aim: To determine the operating characteristics of an IgG subclass in IIF. Methods: We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies. Results: The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were > 97%. Conclusion: Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.

Original languageEnglish
JournalClinical and Experimental Dermatology
Volume43
Issue number3
Pages (from-to)248-253
Number of pages6
ISSN0307-6938
DOIs
Publication statusPublished - 04.2018

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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