Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses

Gereon Hüttmann; Benno Radt; Jesper Serbin; Reginald Birngruber

doi:10.1117/12.500525

Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses

Gereon Hüttmann^*, Benno Radt, Jesper Serbin, Reginald Birngruber

^*Corresponding author for this work

Institute of Biomedical Optics

Abstract

Irradiation of 15 nm gold particles with nano- and picosecond laser pulses can create locally temperatures beyond the critical point of water. Due to the short heating times the temperature is localised to the vicinity of the particles. Under irradiation with nano- and picosecond pulses an inactivation of the enzymes alkaline phosphatase and chymotrypsin which were bound to the surface of the particles was observed. As expected by strong temperature gradient caused by the short irradiation time the protein inactivation is localized within a few tens of nanometers when picosecond pulses were used. This was shown by irradiating gold protein conjugates in which the protein was bound via two antibodies to the particle.

Original language	English
Title of host publication	Therapeutic Laser Applications and Laser-Tissue Interactions
Editors	Rudolf W. Steiner
Number of pages	8
Publisher	SPIE
Publication date	16.10.2003
Pages	88-95
DOIs	https://doi.org/10.1117/12.500525
Publication status	Published - 16.10.2003
Event	Therapeutic Laser Applications and Laser-Tissue Interactions 2003 - Munich, Germany Duration: 24.06.2003 → 25.06.2003 Conference number: 62305

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Academic Focus: Biomedical Engineering

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Hüttmann, G., Radt, B., Serbin, J., & Birngruber, R. (2003). Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses. In R. W. Steiner (Ed.), Therapeutic Laser Applications and Laser-Tissue Interactions (pp. 88-95). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 5142). SPIE. https://doi.org/10.1117/12.500525

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title = "Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses",

abstract = "Irradiation of 15 nm gold particles with nano- and picosecond laser pulses can create locally temperatures beyond the critical point of water. Due to the short heating times the temperature is localised to the vicinity of the particles. Under irradiation with nano- and picosecond pulses an inactivation of the enzymes alkaline phosphatase and chymotrypsin which were bound to the surface of the particles was observed. As expected by strong temperature gradient caused by the short irradiation time the protein inactivation is localized within a few tens of nanometers when picosecond pulses were used. This was shown by irradiating gold protein conjugates in which the protein was bound via two antibodies to the particle.",

author = "Gereon H{\"u}ttmann and Benno Radt and Jesper Serbin and Reginald Birngruber",

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Hüttmann, G, Radt, B, Serbin, J & Birngruber, R 2003, Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses. in R W. Steiner (ed.), Therapeutic Laser Applications and Laser-Tissue Interactions. Proceedings of SPIE - The International Society for Optical Engineering, vol. 5142, SPIE, pp. 88-95, Therapeutic Laser Applications and Laser-Tissue Interactions 2003, Munich, Bavaria, Germany, 24.06.03. https://doi.org/10.1117/12.500525

Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses. / Hüttmann, Gereon; Radt, Benno; Serbin, Jesper et al.
Therapeutic Laser Applications and Laser-Tissue Interactions. ed. / Rudolf W. Steiner. SPIE, 2003. p. 88-95 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 5142).

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

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T1 - Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses

AU - Hüttmann, Gereon

AU - Radt, Benno

AU - Serbin, Jesper

AU - Birngruber, Reginald

N1 - Conference code: 62305

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N2 - Irradiation of 15 nm gold particles with nano- and picosecond laser pulses can create locally temperatures beyond the critical point of water. Due to the short heating times the temperature is localised to the vicinity of the particles. Under irradiation with nano- and picosecond pulses an inactivation of the enzymes alkaline phosphatase and chymotrypsin which were bound to the surface of the particles was observed. As expected by strong temperature gradient caused by the short irradiation time the protein inactivation is localized within a few tens of nanometers when picosecond pulses were used. This was shown by irradiating gold protein conjugates in which the protein was bound via two antibodies to the particle.

AB - Irradiation of 15 nm gold particles with nano- and picosecond laser pulses can create locally temperatures beyond the critical point of water. Due to the short heating times the temperature is localised to the vicinity of the particles. Under irradiation with nano- and picosecond pulses an inactivation of the enzymes alkaline phosphatase and chymotrypsin which were bound to the surface of the particles was observed. As expected by strong temperature gradient caused by the short irradiation time the protein inactivation is localized within a few tens of nanometers when picosecond pulses were used. This was shown by irradiating gold protein conjugates in which the protein was bound via two antibodies to the particle.

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M3 - Conference contribution

T3 - Proceedings of SPIE - The International Society for Optical Engineering

SP - 88

EP - 95

BT - Therapeutic Laser Applications and Laser-Tissue Interactions

A2 - W. Steiner, Rudolf

PB - SPIE

T2 - Therapeutic Laser Applications and Laser-Tissue Interactions 2003

Y2 - 24 June 2003 through 25 June 2003

ER -

Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses

Abstract

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