In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence

Regina Orzekowsky-Schroeder, Antje Klinger, Björn Martensen, Maike Blessenohl, Andreas Gebert, Alfred Vogel, Gereon Hüttmann*

*Corresponding author for this work
23 Citations (Scopus)

Abstract

Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.

Original languageEnglish
Article number116025
JournalJournal of Biomedical Optics
Volume16
Issue number11
ISSN1083-3668
DOIs
Publication statusPublished - 01.11.2011

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