In vivo protein crystallization opens new routes in structural biology

Rudolf Koopmann; Karolina Cupelli; Lars Redecke; Karol Nass; Daniel P. Deponte; Thomas A. White; Francesco Stellato; Dirk Rehders; Mengning Liang; Jakob Andreasson; Andrew Aquila; Sasa Bajt; Miriam Barthelmess; Anton Barty; Michael J. Bogan; Christoph Bostedt; Sébastien Boutet; John D. Bozek; Carl Caleman; Nicola Coppola; Jan Davidsson; R. Bruce Doak; Tomas Ekeberg; Sascha W. Epp; Benjamin Erk; Holger Fleckenstein; Lutz Foucar; Heinz Graafsma; Lars Gumprecht; Janos Hajdu; Christina Y. Hampton; Andreas Hartmann; Robert Hartmann; Gülnter Hauser; Helmut Hirsemann; Peter Holl; Mark S. Hunter; Stephan Kassemeyer; Richard A. Kirian; Lukas Lomb; Filipe R.N.C. Maia; Nils Kimmel; Andrew V. Martin; Marc Messerschmidt; Christian Reich; Daniel Rolles; Benedikt Rudek; Artem Rudenko; Ilme Schlichting; Joachim Schulz; M. Marvin Seibert; Robert L. Shoeman; Raymond G. Sierra; Heike Soltau; Stephan Stern; Lothar Strülder; Nicusor Timneanu; Joachim Ullrich; Xiaoyu Wang; Georg Weidenspointner; Uwe Weierstall; Garth J. Williams; Cornelia B. Wunderer; Petra Fromme; John C.H. Spence; Thilo Stehle; Henry N. Chapman; Christian Betzel; Michael Duszenko

doi:10.1038/nmeth.1859

In vivo protein crystallization opens new routes in structural biology

Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P. Deponte, Thomas A. White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, Sébastien Boutet, John D. Bozek, Carl Caleman, Nicola CoppolaJan Davidsson, R. Bruce Doak, Tomas Ekeberg, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Gülnter Hauser, Helmut Hirsemann, Peter Holl, Mark S. Hunter, Stephan Kassemeyer, Richard A. Kirian, Lukas Lomb, Filipe R.N.C. Maia, Nils Kimmel, Andrew V. Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond G. Sierra, Heike Soltau, Stephan Stern, Lothar Strülder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J. Williams, Cornelia B. Wunderer, Petra Fromme, John C.H. Spence, Thilo Stehle, Henry N. Chapman, Christian Betzel, Michael Duszenko^*

^*Corresponding author for this work

Institute of Biochemistry

104 Citations (Scopus)

Abstract

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Original language	English
Journal	Nature Methods
Volume	9
Issue number	3
Pages (from-to)	259-262
Number of pages	4
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.1859
Publication status	Published - 01.03.2012

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10.1038/nmeth.1859

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Koopmann, R., Cupelli, K., Redecke, L., Nass, K., Deponte, D. P., White, T. A., Stellato, F., Rehders, D., Liang, M., Andreasson, J., Aquila, A., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., Bostedt, C., Boutet, S., Bozek, J. D., Caleman, C., ... Duszenko, M. (2012). In vivo protein crystallization opens new routes in structural biology. Nature Methods, 9(3), 259-262. https://doi.org/10.1038/nmeth.1859

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title = "In vivo protein crystallization opens new routes in structural biology",

abstract = "Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.",

author = "Rudolf Koopmann and Karolina Cupelli and Lars Redecke and Karol Nass and Deponte, {Daniel P.} and White, {Thomas A.} and Francesco Stellato and Dirk Rehders and Mengning Liang and Jakob Andreasson and Andrew Aquila and Sasa Bajt and Miriam Barthelmess and Anton Barty and Bogan, {Michael J.} and Christoph Bostedt and S{\'e}bastien Boutet and Bozek, {John D.} and Carl Caleman and Nicola Coppola and Jan Davidsson and Doak, {R. Bruce} and Tomas Ekeberg and Epp, {Sascha W.} and Benjamin Erk and Holger Fleckenstein and Lutz Foucar and Heinz Graafsma and Lars Gumprecht and Janos Hajdu and Hampton, {Christina Y.} and Andreas Hartmann and Robert Hartmann and G{\"u}lnter Hauser and Helmut Hirsemann and Peter Holl and Hunter, {Mark S.} and Stephan Kassemeyer and Kirian, {Richard A.} and Lukas Lomb and Maia, {Filipe R.N.C.} and Nils Kimmel and Martin, {Andrew V.} and Marc Messerschmidt and Christian Reich and Daniel Rolles and Benedikt Rudek and Artem Rudenko and Ilme Schlichting and Joachim Schulz and Seibert, {M. Marvin} and Shoeman, {Robert L.} and Sierra, {Raymond G.} and Heike Soltau and Stephan Stern and Lothar Str{\"u}lder and Nicusor Timneanu and Joachim Ullrich and Xiaoyu Wang and Georg Weidenspointner and Uwe Weierstall and Williams, {Garth J.} and Wunderer, {Cornelia B.} and Petra Fromme and Spence, {John C.H.} and Thilo Stehle and Chapman, {Henry N.} and Christian Betzel and Michael Duszenko",

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Koopmann, R, Cupelli, K, Redecke, L, Nass, K, Deponte, DP, White, TA, Stellato, F, Rehders, D, Liang, M, Andreasson, J, Aquila, A, Bajt, S, Barthelmess, M, Barty, A, Bogan, MJ, Bostedt, C, Boutet, S, Bozek, JD, Caleman, C, Coppola, N, Davidsson, J, Doak, RB, Ekeberg, T, Epp, SW, Erk, B, Fleckenstein, H, Foucar, L, Graafsma, H, Gumprecht, L, Hajdu, J, Hampton, CY, Hartmann, A, Hartmann, R, Hauser, G, Hirsemann, H, Holl, P, Hunter, MS, Kassemeyer, S, Kirian, RA, Lomb, L, Maia, FRNC, Kimmel, N, Martin, AV, Messerschmidt, M, Reich, C, Rolles, D, Rudek, B, Rudenko, A, Schlichting, I, Schulz, J, Seibert, MM, Shoeman, RL, Sierra, RG, Soltau, H, Stern, S, Strülder, L, Timneanu, N, Ullrich, J, Wang, X, Weidenspointner, G, Weierstall, U, Williams, GJ, Wunderer, CB, Fromme, P, Spence, JCH, Stehle, T, Chapman, HN, Betzel, C & Duszenko, M 2012, 'In vivo protein crystallization opens new routes in structural biology', Nature Methods, vol. 9, no. 3, pp. 259-262. https://doi.org/10.1038/nmeth.1859

TY - JOUR

T1 - In vivo protein crystallization opens new routes in structural biology

AU - Koopmann, Rudolf

AU - Cupelli, Karolina

AU - Redecke, Lars

AU - Nass, Karol

AU - Deponte, Daniel P.

AU - White, Thomas A.

AU - Stellato, Francesco

AU - Rehders, Dirk

AU - Liang, Mengning

AU - Andreasson, Jakob

AU - Aquila, Andrew

AU - Bajt, Sasa

AU - Barthelmess, Miriam

AU - Barty, Anton

AU - Bogan, Michael J.

AU - Bostedt, Christoph

AU - Boutet, Sébastien

AU - Bozek, John D.

AU - Caleman, Carl

AU - Coppola, Nicola

AU - Davidsson, Jan

AU - Doak, R. Bruce

AU - Ekeberg, Tomas

AU - Epp, Sascha W.

AU - Erk, Benjamin

AU - Fleckenstein, Holger

AU - Foucar, Lutz

AU - Graafsma, Heinz

AU - Gumprecht, Lars

AU - Hajdu, Janos

AU - Hampton, Christina Y.

AU - Hartmann, Andreas

AU - Hartmann, Robert

AU - Hauser, Gülnter

AU - Hirsemann, Helmut

AU - Holl, Peter

AU - Hunter, Mark S.

AU - Kassemeyer, Stephan

AU - Kirian, Richard A.

AU - Lomb, Lukas

AU - Maia, Filipe R.N.C.

AU - Kimmel, Nils

AU - Martin, Andrew V.

AU - Messerschmidt, Marc

AU - Reich, Christian

AU - Rolles, Daniel

AU - Rudek, Benedikt

AU - Rudenko, Artem

AU - Schlichting, Ilme

AU - Schulz, Joachim

AU - Seibert, M. Marvin

AU - Shoeman, Robert L.

AU - Sierra, Raymond G.

AU - Soltau, Heike

AU - Stern, Stephan

AU - Strülder, Lothar

AU - Timneanu, Nicusor

AU - Ullrich, Joachim

AU - Wang, Xiaoyu

AU - Weidenspointner, Georg

AU - Weierstall, Uwe

AU - Williams, Garth J.

AU - Wunderer, Cornelia B.

AU - Fromme, Petra

AU - Spence, John C.H.

AU - Stehle, Thilo

AU - Chapman, Henry N.

AU - Betzel, Christian

AU - Duszenko, Michael

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

AB - Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

UR - http://www.scopus.com/inward/record.url?scp=84862778001&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1859

DO - 10.1038/nmeth.1859

M3 - Journal articles

C2 - 22286384

AN - SCOPUS:84862778001

SN - 1548-7091

VL - 9

SP - 259

EP - 262

JO - Nature Methods

JF - Nature Methods

IS - 3

ER -

In vivo protein crystallization opens new routes in structural biology

Abstract

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