In vivo protein crystallization opens new routes in structural biology

Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P. Deponte, Thomas A. White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, Sébastien Boutet, John D. Bozek, Carl Caleman, Nicola CoppolaJan Davidsson, R. Bruce Doak, Tomas Ekeberg, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Gülnter Hauser, Helmut Hirsemann, Peter Holl, Mark S. Hunter, Stephan Kassemeyer, Richard A. Kirian, Lukas Lomb, Filipe R.N.C. Maia, Nils Kimmel, Andrew V. Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond G. Sierra, Heike Soltau, Stephan Stern, Lothar Strülder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J. Williams, Cornelia B. Wunderer, Petra Fromme, John C.H. Spence, Thilo Stehle, Henry N. Chapman, Christian Betzel, Michael Duszenko*

*Corresponding author for this work
104 Citations (Scopus)


Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Original languageEnglish
JournalNature Methods
Issue number3
Pages (from-to)259-262
Number of pages4
Publication statusPublished - 01.03.2012


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