In vivo effects of repeated thyronamine administration in male C57BL/6J mice

Lisbeth Harder, Nancy Schanze, Assel Sarsenbayeva, Franziska Kugel, Josef Köhrle, Lutz Schomburg, Jens Mittag*, Carolin S. Hoefig

*Corresponding author for this work
3 Citations (Scopus)

Abstract

Objectives: Thyronamines are decarboxylated and deiodinated metabolites of thyroid hormones (THs). Of all possible thyronamine variants, only 3-iodothyronamine (3-T1AM) and iodine-free thyronamine (T0AM) have been detected in vivo. While intensive research has been done on the (patho-) physiological action of 3-T1AM, the role of T0AM has been studied less intensively. Study Design: We determined whether a single pharmacological dose (50 mg/kg, i.p.) or repeated administration (5 mg/kg/day, i.p., for 7 days) of T0AM affects metabolism, cardiovascular function, or thermoregulation in male C57BL/6J mice. Since selenium (Se) is important for proper TH function and Se metabolism is affected by TH, we additionally analyzed Se concentrations in liver, serum, and kidney using total reflection X-ray analysis. Results: A single injection of T0AM had no effect on heart rate, temperature, or activity as assessed by radio telemetry. Likewise, daily administration of T0AM did not alter body weight, food or water intake, heart rate, blood pressure, brown adipose tissue thermogenesis, or body temperature, and no significant differences in hepatic glycogen content or mRNA expression of genes involved in cardiovascular function or metabolic control were determined. Also, the Xray analysis of Se concentrations revealed no significant changes. However, hepatic T0AM was significantly increased in the treated animals. Conclusions: In summary, our data demonstrate that T0AM elicits no obvious metabolic, cardiovascular, or thermoregulatory activities in mice. As T0AM does also not interfere with TH or Se metabolism, we conclude that the deiodination of 3-T1AM to T0AM constitutes an efficient inactivation mechanism, terminating the actions of the more powerful precursor.

Original languageEnglish
JournalEuropean Thyroid Journal
Volume7
Issue number1
Pages (from-to)3-12
Number of pages10
ISSN2235-0640
DOIs
Publication statusPublished - 01.01.2018

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Coronavirus related work

  • Research on SARS-CoV-2 / COVID-19

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