Improved technique for cannulation of the murine thoracic duct: A valuable tool for the dissection of immune responses

M. Ionac, T. Laskay, D. Labahn, G. Geisslinger, W. Solbach*

*Corresponding author for this work
15 Citations (Scopus)

Abstract

Several experimental approaches have been used in the past for the cannulation of the thoracic duct in mice. Most, however, are characterized by a modest reproducibility and a low lymph yield. Here, we describe a cannulation technique modified with respect to the anesthesia, the use of a silicone cannula and a simple and efficient intraabdominal fixation of the drain. Surgery averaged 45 min with an intra- and postoperative mortality rate of zero. Postoperatively, mice were given access to an exercise wheel allowing increased mobility and consequently a good lymph flow, thus maintaining the function of the cannula. The mice yielded a mean of 29.3 ml/24 h (range 8-40 ml) thoracic duct lymph, which contained a mean of 2.2 x 106 lymphocytes/ml during the first 24 h, decreasing to 0.1 x 106 lymphocytes/ml on the 2nd day after cannulation. Patency of the cannulae was 100% after 3 days. Interestingly, we have detected strain dependent differences in the anatomy of the thoracic duct in the mouse and these need to be considered when cannulation procedures are attempted.

Original languageEnglish
JournalJournal of Immunological Methods
Volume202
Issue number1
Pages (from-to)35-40
Number of pages6
ISSN0022-1759
DOIs
Publication statusPublished - 10.03.1997

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

DFG Research Classification Scheme

  • 204-05 Immunology

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