Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels

Daniel Schraivogel; Susann Gaby Schindler; Johannes Danner; Elisabeth Kremmer; Janina Pfaff; Stefan Hannus; Reinhard Depping; Gunter Meister

doi:10.1093/nar/gkv705

Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels

Daniel Schraivogel, Susann Gaby Schindler, Johannes Danner, Elisabeth Kremmer, Janina Pfaff, Stefan Hannus, Reinhard Depping, Gunter Meister^*

^*Corresponding author for this work

Institute of Physiology

29 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well. Their detailed nuclear functions, however, remain elusive. Furthermore, it is not clear which import routes Ago and TNRC6 proteins take into the nucleus. Using different nuclear transport assays, we find that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus. While import receptors might function redundantly to transport Ago2, we demonstrate that TNRC6 proteins are imported by the Importin-β pathway. Finally, we show that nuclear localization of both Ago2 and TNRC6 proteins can depend on each other suggesting actively balanced cytoplasmic Ago-TNRC6 levels.

Original language	English
Journal	Nucleic Acids Research
Volume	43
Issue number	15
Pages (from-to)	7447-7461
Number of pages	15
ISSN	0305-1048
DOIs	https://doi.org/10.1093/nar/gkv705
Publication status	Published - 01.07.2015

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/nar/gkv705

Cite this

@article{0759d2cd14954b278affb447fa4fc11e,

title = "Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels",

abstract = "MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well. Their detailed nuclear functions, however, remain elusive. Furthermore, it is not clear which import routes Ago and TNRC6 proteins take into the nucleus. Using different nuclear transport assays, we find that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus. While import receptors might function redundantly to transport Ago2, we demonstrate that TNRC6 proteins are imported by the Importin-β pathway. Finally, we show that nuclear localization of both Ago2 and TNRC6 proteins can depend on each other suggesting actively balanced cytoplasmic Ago-TNRC6 levels.",

author = "Daniel Schraivogel and Schindler, {Susann Gaby} and Johannes Danner and Elisabeth Kremmer and Janina Pfaff and Stefan Hannus and Reinhard Depping and Gunter Meister",

year = "2015",

month = jul,

day = "1",

doi = "10.1093/nar/gkv705",

language = "English",

volume = "43",

pages = "7447--7461",

journal = "Nucleic Acids Research",

issn = "0305-1048",

number = "15",

}

TY - JOUR

T1 - Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels

AU - Schraivogel, Daniel

AU - Schindler, Susann Gaby

AU - Danner, Johannes

AU - Kremmer, Elisabeth

AU - Pfaff, Janina

AU - Hannus, Stefan

AU - Depping, Reinhard

AU - Meister, Gunter

PY - 2015/7/1

Y1 - 2015/7/1

N2 - MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well. Their detailed nuclear functions, however, remain elusive. Furthermore, it is not clear which import routes Ago and TNRC6 proteins take into the nucleus. Using different nuclear transport assays, we find that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus. While import receptors might function redundantly to transport Ago2, we demonstrate that TNRC6 proteins are imported by the Importin-β pathway. Finally, we show that nuclear localization of both Ago2 and TNRC6 proteins can depend on each other suggesting actively balanced cytoplasmic Ago-TNRC6 levels.

AB - MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well. Their detailed nuclear functions, however, remain elusive. Furthermore, it is not clear which import routes Ago and TNRC6 proteins take into the nucleus. Using different nuclear transport assays, we find that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus. While import receptors might function redundantly to transport Ago2, we demonstrate that TNRC6 proteins are imported by the Importin-β pathway. Finally, we show that nuclear localization of both Ago2 and TNRC6 proteins can depend on each other suggesting actively balanced cytoplasmic Ago-TNRC6 levels.

UR - http://www.scopus.com/inward/record.url?scp=84941588202&partnerID=8YFLogxK

U2 - 10.1093/nar/gkv705

DO - 10.1093/nar/gkv705

M3 - Journal articles

C2 - 26170235

AN - SCOPUS:84941588202

SN - 0305-1048

VL - 43

SP - 7447

EP - 7461

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 15

ER -

Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels

Abstract

Research Areas and Centers

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this