Implications of RNA structure on the annealing of a potent antisense RNA directed against the human immunodeficiency virus type 1

Sigrid Eckardt, Pascale Romby, Georg Sczakiel*

*Corresponding author for this work
23 Citations (Scopus)

Abstract

Antisense RNA-mediated regulation in bacterial systems is related to the kinetics of RNA-RNA annealing in vitro. Here, we investigated the secondary structure of αY69, an effective HIV-directed antisense RNA in human cells. Purified RNA preparations contain a single conformer. The global structure was identified by a cleavage experiment under native conditions using a short complementary oligonucleotide and RNase H. Structural analyses indicate a three-domain structure of αY69 consisting of two stem-loop elements connected by a seven-nucleotide single-stranded hinge region. Kinetic data suggest that the formation of base pairs between a CGC triplet of αY69 and its target RNA is essential for fast annealing. The complementary sequence stretch of the target folds into a high-energy secondary structure. The relationship between modifications in structural elements of αY69 and the annealing kinetics suggested that rate-limiting steps of the annealing involve a single site of αY69 and do not involve its 5' or 3'-end. Further, the data indicate that both initial base-specific interactions and duplex formation are dependent on the CGC triplet of the central region of αY69. This mechanism represents a specific and efficient way of RNA-RNA annealing that is initiated by the interaction of unstructured RNA regions.

Original languageEnglish
JournalBiochemistry
Volume36
Issue number42
Pages (from-to)12711-12721
Number of pages11
ISSN0006-2960
DOIs
Publication statusPublished - 21.10.1997

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