Immunocytochemical localization of vigilin, a tRNA-binding protein, after cell fractionation and within the exocrine pancreatic cell of the rat

Matthias H.F. Klinger; Charli Kruse

doi:10.1016/S0940-9602(96)80086-0

Immunocytochemical localization of vigilin, a tRNA-binding protein, after cell fractionation and within the exocrine pancreatic cell of the rat

Matthias H.F. Klinger^*, Charli Kruse

^*Corresponding author for this work

17 Citations (Scopus)

Abstract

A polyclonal antibody against a fusion protein composed of two vigilin domains and γ-galactosidase was used to localize the tRNA-binding protein vigilin at the ultrastructural level. Immunoreactivity for vigilin was found on membranes bearing ribosomes within the microsome fraction derived from rat livers and on the rough endoplasmic reticulum in rat exocrine pancreatic cells, thus corroborating the involvement of vigilin in protein synthesis. Gold labeling was also found in the nucleus and in the microsome fraction in an osmiophilic substance resembling heterochromatin. Both of these localizations, the rough endoplasmic reticulum and the nucleus, are in line with the observation that a vigilin-containing ribonucleoprotein complex binds tRNA.

Original language	English
Journal	Annals of Anatomy
Volume	178
Issue number	4
Pages (from-to)	331-335
Number of pages	5
ISSN	0940-9602
DOIs	https://doi.org/10.1016/S0940-9602(96)80086-0
Publication status	Published - 1996

Funding

Acknowledgements. The skilful work of Kerstin Fibelkorn, Emel Klink, and Sonja Markmann is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (DFG - Kr 151211-1).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1016/S0940-9602(96)80086-0

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title = "Immunocytochemical localization of vigilin, a tRNA-binding protein, after cell fractionation and within the exocrine pancreatic cell of the rat",

abstract = "A polyclonal antibody against a fusion protein composed of two vigilin domains and γ-galactosidase was used to localize the tRNA-binding protein vigilin at the ultrastructural level. Immunoreactivity for vigilin was found on membranes bearing ribosomes within the microsome fraction derived from rat livers and on the rough endoplasmic reticulum in rat exocrine pancreatic cells, thus corroborating the involvement of vigilin in protein synthesis. Gold labeling was also found in the nucleus and in the microsome fraction in an osmiophilic substance resembling heterochromatin. Both of these localizations, the rough endoplasmic reticulum and the nucleus, are in line with the observation that a vigilin-containing ribonucleoprotein complex binds tRNA.",

author = "Klinger, \{Matthias H.F.\} and Charli Kruse",

note = "Funding Information: Acknowledgements. The skilful work of Kerstin Fibelkorn, Emel Klink, and Sonja Markmann is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (DFG - Kr 151211-1). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "1996",

doi = "10.1016/S0940-9602(96)80086-0",

language = "English",

volume = "178",

pages = "331--335",

journal = "Annals of Anatomy",

issn = "0940-9602",

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TY - JOUR

T1 - Immunocytochemical localization of vigilin, a tRNA-binding protein, after cell fractionation and within the exocrine pancreatic cell of the rat

AU - Klinger, Matthias H.F.

AU - Kruse, Charli

N1 - Funding Information: Acknowledgements. The skilful work of Kerstin Fibelkorn, Emel Klink, and Sonja Markmann is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (DFG - Kr 151211-1). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1996

Y1 - 1996

N2 - A polyclonal antibody against a fusion protein composed of two vigilin domains and γ-galactosidase was used to localize the tRNA-binding protein vigilin at the ultrastructural level. Immunoreactivity for vigilin was found on membranes bearing ribosomes within the microsome fraction derived from rat livers and on the rough endoplasmic reticulum in rat exocrine pancreatic cells, thus corroborating the involvement of vigilin in protein synthesis. Gold labeling was also found in the nucleus and in the microsome fraction in an osmiophilic substance resembling heterochromatin. Both of these localizations, the rough endoplasmic reticulum and the nucleus, are in line with the observation that a vigilin-containing ribonucleoprotein complex binds tRNA.

AB - A polyclonal antibody against a fusion protein composed of two vigilin domains and γ-galactosidase was used to localize the tRNA-binding protein vigilin at the ultrastructural level. Immunoreactivity for vigilin was found on membranes bearing ribosomes within the microsome fraction derived from rat livers and on the rough endoplasmic reticulum in rat exocrine pancreatic cells, thus corroborating the involvement of vigilin in protein synthesis. Gold labeling was also found in the nucleus and in the microsome fraction in an osmiophilic substance resembling heterochromatin. Both of these localizations, the rough endoplasmic reticulum and the nucleus, are in line with the observation that a vigilin-containing ribonucleoprotein complex binds tRNA.

UR - https://www.scopus.com/pages/publications/0029856204

U2 - 10.1016/S0940-9602(96)80086-0

DO - 10.1016/S0940-9602(96)80086-0

M3 - Journal articles

C2 - 8817039

AN - SCOPUS:0029856204

SN - 0940-9602

VL - 178

SP - 331

EP - 335

JO - Annals of Anatomy

JF - Annals of Anatomy

IS - 4

ER -

Immunocytochemical localization of vigilin, a tRNA-binding protein, after cell fractionation and within the exocrine pancreatic cell of the rat

Abstract

Funding

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