Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid

Michael Mersmann; Jenny Dworschak; Kristin Ebermann; Lars Komorowski; Wolfgang Schlumberger; Winfried Stöcker; Detlef Zillikens; Christian Probst; Enno Schmidt

doi:10.1007/s00403-015-1606-7

Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid

Michael Mersmann^*, Jenny Dworschak, Kristin Ebermann, Lars Komorowski, Wolfgang Schlumberger, Winfried Stöcker, Detlef Zillikens, Christian Probst, Enno Schmidt

^*Corresponding author for this work

Euroimmun AG

15 Citations (Scopus)

Abstract

Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.

Original language	English
Journal	Archives of Dermatological Research
Volume	308
Issue number	1
Pages (from-to)	31-38
Number of pages	8
ISSN	0340-3696
DOIs	https://doi.org/10.1007/s00403-015-1606-7
Publication status	Published - 01.01.2016

Funding

This work was supported by the European Regional Development Fund (project no. 122-09-017) and the Excellence Cluster Inflammation@Interfaces (DFG EXC306/1 and 2).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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title = "Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid",

abstract = "Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.",

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T1 - Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid

AU - Mersmann, Michael

AU - Dworschak, Jenny

AU - Ebermann, Kristin

AU - Komorowski, Lars

AU - Schlumberger, Wolfgang

AU - Stöcker, Winfried

AU - Zillikens, Detlef

AU - Probst, Christian

AU - Schmidt, Enno

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.

AB - Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.

UR - https://www.scopus.com/pages/publications/84954392492

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SN - 0340-3696

VL - 308

SP - 31

EP - 38

JO - Archives of Dermatological Research

JF - Archives of Dermatological Research

IS - 1

ER -

Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid

Abstract

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UN SDGs

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