Immundiagnostischer stellenwert der antigenspezifischen ELISAs in der ANCA-diagnostik bei primaren systemischen vaskulitiden

Translated title of the contribution: Diagnostic value of ANCA antigen testing in primary systemic vasculitides

K. de Groot, E. Csernok*, G. Wang, W. L. Gross

*Corresponding author for this work


The indirect immunofluorescence test (IFT) is used as a screening method for anti-neutrophil cytoplasmic antibodies (ANCA) as diagnostic markers for systemic vasculitides. The sensitivity and specificity of ANCA antigen detection (proteinase 3 (PR3) and myeloperoxidase (MPO)) by ELISA differs considerably between centers. We assessed commercial ELISA kits in respect to their specificity and sensitivity in detecting PR3-ANCA (7 kits) and MPO-ANCA (8 kits) and their correlation with the IFT results. Sera from 5 patients with systemic lupus erythematodes (SLE), 28 with Wegener's granulomatosis (WG), 22 with microscopic polyangiitis (MPA), 5 with idiopathic rapidly progressive glomerulonephritis (RPGN), and 5 healthy controls were examined by IFT and commercial ELISA kits. Sera from healthy controls, patients with SLE and cANCA-negative WG were shown to be PR3-ANCA-negative by all 7 PR3- ANCA kits. In 25 cANCA-positive sera from WG patients, PR3-ANCA positivity ranges from 44% to 84%. The PR3-ANCA levels in 5 of 7 kits correlated with the cANCA titers in IFT. Sera from healthy controls, 4/5 SLE and all pANCA- negative SLE patients were found to be MPO-ANCA-negative in all 8 MPO-ANCA kits. In 20 pANCA-positive sera, MPO positivity ranged from 25% to 75%. Only 35% of the MPO-ANCA-positive sera could be confirmed by other immunological methods. No significant correlation was observed between pANCA titers and MPO-ANCA levels. The PR3 and MPO-ANCA ELISA kits under investigation show a great variability concerning their sensitivity and in case of MPO concerning their specificity, too. So far, the method of choice for ANCA screening remains the IFT.

Translated title of the contributionDiagnostic value of ANCA antigen testing in primary systemic vasculitides
Original languageGerman
JournalNieren- und Hochdruckkrankheiten
Issue number7
Pages (from-to)247-253
Number of pages7
Publication statusPublished - 07.1999

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


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