TY - JOUR
T1 - Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins
AU - Barnwal, Ravi Pratap
AU - Rout, Ashok K
AU - Atreya, Hanudatta S
AU - Chary, Kandala V R
PY - 2008/8
Y1 - 2008/8
N2 - We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.
AB - We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.
U2 - 10.1007/s10858-008-9254-2
DO - 10.1007/s10858-008-9254-2
M3 - Scientific review articles
C2 - 18633715
SN - 0925-2738
VL - 41
SP - 191
EP - 197
JO - Journal of Biomolecular NMR
JF - Journal of Biomolecular NMR
IS - 4
ER -
