Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins

Ravi Pratap Barnwal; Ashok K Rout; Hanudatta S Atreya; Kandala V R Chary

doi:10.1007/s10858-008-9254-2

Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins

Ravi Pratap Barnwal, Ashok K Rout, Hanudatta S Atreya, Kandala V R Chary

Institute of Chemistry and Metabolomics

Tata Institute of Fundamental Research

25 Citations (Scopus)

Abstract

We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.

Original language	English
Journal	Journal of Biomolecular NMR
Volume	41
Issue number	4
Pages (from-to)	191-7
Number of pages	7
ISSN	0925-2738
DOIs	https://doi.org/10.1007/s10858-008-9254-2
Publication status	Published - 08.2008

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10.1007/s10858-008-9254-2

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abstract = "We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.",

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T1 - Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins

AU - Barnwal, Ravi Pratap

AU - Rout, Ashok K

AU - Atreya, Hanudatta S

AU - Chary, Kandala V R

PY - 2008/8

Y1 - 2008/8

N2 - We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.

AB - We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.

U2 - 10.1007/s10858-008-9254-2

DO - 10.1007/s10858-008-9254-2

M3 - Scientific review articles

C2 - 18633715

SN - 0925-2738

VL - 41

SP - 191

EP - 197

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 4

ER -

Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins

Abstract

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