Identification of a tRNA-specific nuclear export receptor

Ulrike Kutay; Gerd Lipowsky; Elisa Izaurralde; F. Ralf Bischoff; Petra Schwarzmaier; Enno Hartmann; Dirk Görlich

doi:10.1016/S1097-2765(00)80036-2

Identification of a tRNA-specific nuclear export receptor

Ulrike Kutay, Gerd Lipowsky, Elisa Izaurralde, F. Ralf Bischoff, Petra Schwarzmaier, Enno Hartmann, Dirk Görlich^*

^*Corresponding author for this work

Institute of Biology

266 Citations (Scopus)

Abstract

In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin β-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.

Original language	English
Journal	Molecular Cell
Volume	1
Issue number	3
Pages (from-to)	359-369
Number of pages	11
ISSN	1097-2765
DOIs	https://doi.org/10.1016/S1097-2765(00)80036-2
Publication status	Published - 01.01.1998

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abstract = "In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin β-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.",

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T1 - Identification of a tRNA-specific nuclear export receptor

AU - Kutay, Ulrike

AU - Lipowsky, Gerd

AU - Izaurralde, Elisa

AU - Bischoff, F. Ralf

AU - Schwarzmaier, Petra

AU - Hartmann, Enno

AU - Görlich, Dirk

PY - 1998/1/1

Y1 - 1998/1/1

N2 - In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin β-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.

AB - In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin β-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.

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AN - SCOPUS:0031990346

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JO - Molecular Cell

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Identification of a tRNA-specific nuclear export receptor

Abstract

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