Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Karola Rittner; George Sczakiel

doi:10.1093/nar/19.7.1421

Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Karola Rittner, George Sczakiel^*

^*Corresponding author for this work

German Cancer Research Center

62 Citations (Scopus)

Abstract

Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

Original language	English
Journal	Nucleic Acids Research
Volume	19
Issue number	7
Pages (from-to)	1421-1426
Number of pages	6
ISSN	0305-1048
DOIs	https://doi.org/10.1093/nar/19.7.1421
Publication status	Published - 11.04.1991

Funding

We thank H. zur Hausen for continuous support, M. Pawlita for critical discussion and A. Kleinheinz for helpful advice for the microinjection experiments. We also thank V. Bosch for critically reading this manuscript. This work was supported in part by BMFT grant FKZ-II-083-89.

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10.1093/nar/19.7.1421

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abstract = "Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.",

author = "Karola Rittner and George Sczakiel",

note = "Funding Information: We thank H. zur Hausen for continuous support, M. Pawlita for critical discussion and A. Kleinheinz for helpful advice for the microinjection experiments. We also thank V. Bosch for critically reading this manuscript. This work was supported in part by BMFT grant FKZ-II-083-89.",

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doi = "10.1093/nar/19.7.1421",

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AU - Rittner, Karola

AU - Sczakiel, George

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N2 - Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

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Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Abstract

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