Hypoxia enhances prostaglandin synthesis in renal mesangial cell cultures

Wolfgang Jelkmann; Armin Kurtz; Ulrich Förstermann; Josef Pfeilschifter; Christian Bauer

doi:10.1016/S0090-6980(85)80014-9

Hypoxia enhances prostaglandin synthesis in renal mesangial cell cultures

Wolfgang Jelkmann^*, Armin Kurtz, Ulrich Förstermann, Josef Pfeilschifter, Christian Bauer

^*Corresponding author for this work

Institute of Physiology

16 Citations (Scopus)

Abstract

In view of recent findings which suggest that renal prostaglandins mediate the effect of hypoxia on erythropoietin production, we have studied whether hypoxia is a stimulus for in vitro prostaglandin synthesis. Studies were carried out in rat renal mesangial cell cultures which produce erythropoietin in an oxygen-dependent manner. Production rates of PGE₂ and in specified samples also of 6-keto-PGF_1α, as a measure of PGI₂, and PGF_2α were determined by radioimmunoassay after incubation at either 20% O₂ (normoxic) or 2% O₂ (hypoxic) in gas permeable dishes for 24 hrs. Considerable variation in PGE₂ production was noted among independent cell lines. PGE₂ production appeared to be inversely correlated to the cellular density of the cultures. In addition, PGE₂ production was enhanced in hypoxic cell cultures. The mean increase was 50 to 60%. PGF_2α and 6-keto-PGF_1α increased by about the same rate. These results indicate that hypoxia is a stimulus for in vitro prostaglandin production.

Original language	English
Journal	Prostaglandins
Volume	30
Issue number	1
Pages (from-to)	109-118
Number of pages	10
ISSN	0090-6980
DOIs	https://doi.org/10.1016/S0090-6980(85)80014-9
Publication status	Published - 07.1985

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Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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title = "Hypoxia enhances prostaglandin synthesis in renal mesangial cell cultures",

abstract = "In view of recent findings which suggest that renal prostaglandins mediate the effect of hypoxia on erythropoietin production, we have studied whether hypoxia is a stimulus for in vitro prostaglandin synthesis. Studies were carried out in rat renal mesangial cell cultures which produce erythropoietin in an oxygen-dependent manner. Production rates of PGE2 and in specified samples also of 6-keto-PGF1α, as a measure of PGI2, and PGF2α were determined by radioimmunoassay after incubation at either 20% O2 (normoxic) or 2% O2 (hypoxic) in gas permeable dishes for 24 hrs. Considerable variation in PGE2 production was noted among independent cell lines. PGE2 production appeared to be inversely correlated to the cellular density of the cultures. In addition, PGE2 production was enhanced in hypoxic cell cultures. The mean increase was 50 to 60%. PGF2α and 6-keto-PGF1α increased by about the same rate. These results indicate that hypoxia is a stimulus for in vitro prostaglandin production.",

author = "Wolfgang Jelkmann and Armin Kurtz and Ulrich F{\"o}rstermann and Josef Pfeilschifter and Christian Bauer",

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AU - Jelkmann, Wolfgang

AU - Kurtz, Armin

AU - Förstermann, Ulrich

AU - Pfeilschifter, Josef

AU - Bauer, Christian

PY - 1985/7

Y1 - 1985/7

N2 - In view of recent findings which suggest that renal prostaglandins mediate the effect of hypoxia on erythropoietin production, we have studied whether hypoxia is a stimulus for in vitro prostaglandin synthesis. Studies were carried out in rat renal mesangial cell cultures which produce erythropoietin in an oxygen-dependent manner. Production rates of PGE2 and in specified samples also of 6-keto-PGF1α, as a measure of PGI2, and PGF2α were determined by radioimmunoassay after incubation at either 20% O2 (normoxic) or 2% O2 (hypoxic) in gas permeable dishes for 24 hrs. Considerable variation in PGE2 production was noted among independent cell lines. PGE2 production appeared to be inversely correlated to the cellular density of the cultures. In addition, PGE2 production was enhanced in hypoxic cell cultures. The mean increase was 50 to 60%. PGF2α and 6-keto-PGF1α increased by about the same rate. These results indicate that hypoxia is a stimulus for in vitro prostaglandin production.

AB - In view of recent findings which suggest that renal prostaglandins mediate the effect of hypoxia on erythropoietin production, we have studied whether hypoxia is a stimulus for in vitro prostaglandin synthesis. Studies were carried out in rat renal mesangial cell cultures which produce erythropoietin in an oxygen-dependent manner. Production rates of PGE2 and in specified samples also of 6-keto-PGF1α, as a measure of PGI2, and PGF2α were determined by radioimmunoassay after incubation at either 20% O2 (normoxic) or 2% O2 (hypoxic) in gas permeable dishes for 24 hrs. Considerable variation in PGE2 production was noted among independent cell lines. PGE2 production appeared to be inversely correlated to the cellular density of the cultures. In addition, PGE2 production was enhanced in hypoxic cell cultures. The mean increase was 50 to 60%. PGF2α and 6-keto-PGF1α increased by about the same rate. These results indicate that hypoxia is a stimulus for in vitro prostaglandin production.

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Hypoxia enhances prostaglandin synthesis in renal mesangial cell cultures

Abstract

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